Helicoverpa zea Boddie (Lepidoptera: Noctuidae) is an important pest in many crops in the southern United States. Upon reaching the final larval instar, H. zea quests for a pupation site in the soil. Pupae are vulnerable to mortality since their movement is limited. Soil type and moisture can influence H. zea emergence, but the interaction of these factors has not been demonstrated. We compared sandy and clay soils in greenhouse and laboratory experiments. In the first experiment, we evaluated the preference of larvae to choose either sandy or clay soil for pupation. In a second experiment, we set the sandy soils at different moisture levels and observed prepupae pupation preference in a choice scenario. In a third experiment, we observed prepupae pupation in different moisture levels in a no-choice scenario. In a 4th experiment, we evaluated adult emergence following pupation when we increased moisture or kept it constant. In a final experiment, we evaluated pupation behavior in sandy or clay soils with a webcam and a glass arena. We found that larvae preferred to pupate in sandy soils over clay soils and that pupal success was highest at intermediate moisture levels. In addition, elevated soil moisture levels did not impact the emergence of H. zea between sandy or clay soil. Finally, H. zea did not take longer to burrow in either sandy or clay soil, but the tunnels of the pupal burrow were larger in sandy soil compared to clay soil. Our results clarify H. zea behavior across soil moisture and soil type.

Helicoverpa zea (Boddie) is a polyphagous pest that can cause serious damage to crops, including soybeans (Glycine max L.). In soybeans with both determinate and indeterminate growth habits, H. zea larvae are more commonly found on leaves compared to blooms, stems, and pods. Past research demonstrated that H. zea adults tend to oviposit near the upper part of the plant canopy in soybeans with a determinate growth habit. However, ovipositional selection on soybeans with an indeterminate growth habit is unknown. We hypothesized that H. zea would oviposit more uniformly throughout the canopy on indeterminate soybean growth habits due to more diffuse reproductive tissue. We planted field and greenhouse experiments with varieties sharing a similar relative maturity (2 maturity group [MG] 5.2 varieties and 2 MG 5.4/5.5 varieties) but with different growth habits. To test oviposition selection, adult H. zea females were allowed to oviposit under field and caged conditions. We counted the number of H. zea eggs and neonates on each plant tissue type from each of 3 equal parts in the plant canopy: upper, middle, and lower. In both experiments, eggs and neonates were most common on leaves at the top of the plant regardless of soybean growth habit. Consequently, ovipositional selection is likely independent of reproductive tissue availability, and patterns of oviposition through the canopy are similar in growth habits. An improved understanding of H. zea ecology in soybeans relative to indeterminate growth habits may improve recommendations for managing this significant pest of soybean.

Emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), has caused extensive mortality of ash across North America. Biological control offers a potential long-term management option, allowing for long-term survival of ash. Careful monitoring of populations of biocontrol agents is necessary to understand their relative impacts. Understanding the emergence and flight phenology of these species allows for the optimization of monitoring schemes and improves our understanding of host–parasitoid interactions. We used yellow pan trapping data to assess the adult phenology of both EAB and its associated native and introduced parasitoids in 3 NewYork counties. We monitored 2 introduced larval biocontrol agents, Tetrastichus planipennisi Yang (Hymenoptera: Eulophidae) and Spathius galinae Belokobylskij & Strazanac (Braconidae), for 3–4 years post-release, as well as the native parasitoid Phasgonophora sulcata Westword (Chalcididae). Results indicate a single discrete emergence event for both EAB and P. sulcata in all monitored counties, which is consistent with previously reported results. Our results also suggest there are 4 generations per year of T. planipennisi and 3 generations of S. galinae in the monitored counties. We recorded an additional generation of T. planipennisi that had not previously been reported in New York, and both T. planipennisi and S. galinae appeared to emerge earlier than previously documented.

Emerald ash borer (EAB) (Agrilus planipennis Fairmaire) (Coleoptera: Buprestidae) is the most destructive insect to invade North American forests. Identifying habitat features that support EAB natural enemies is necessary to enhance EAB biological control. In many forest ecosystems, tree species diversity has been linked with reduced pest abundance and increases in natural enemy abundance. We assessed the influence of tree species richness, ash density, and proportion of total ash basal area on ash canopy condition, EAB larval densities, and biocontrol by woodpeckers and parasitoids in pairs of healthy and declining overstory (DBH > 10 cm) and recruit-sized ash (DBH 2–10 cm) in 4 post-invasion forests in Michigan, USA. Tree species richness and ash density were not significantly associated with EAB larval densities, ash canopy dieback and transparency, and woodpecker predation of EAB larvae. In declining and healthy overstory ash, woodpeckers killed 38.5 ± 3.9% and 13.2 ± 3.7% of larvae, respectively, while the native parasitoid Phasgonophora sulcata Westwood killed 15.8 ± 3.8% and 8.3 ± 3.0% and the introduced parasitoid Spathius galinae Belokobylskij & Strazanac killed 10.8 ± 2.5% and 5.0 ± 2.6% of EAB larvae. Parasitism by P. sulcata was inversely related to ash density while parasitism by S. galinae was positively associated with ash density. Ash density, but not tree diversity, appears to differentially influence biological control of EAB by parasitoids, but this effect is not associated with reduced EAB densities or improved canopy condition.

Termites are social insects with high species diversity in tropical ecosystems. Multivariate analysis with near-infrared spectroscopy (NIRS) and data interpretation can separate social insects belonging to different colonies of the same species. The objective of this study was to propose the use of discriminant analysis by partial least squares (PLS-DA) combined with NIRS to identify the colonial origin of the Syntermes grandis (Rambur, 1842) (Blattodea: Termitidae) in 2 castes. Six ground S. grandis colonies were identified and mapped; 30 workers and 30 soldier termites in each colony were submitted to spectral measurement with NIRS. PLS-DA applied to the termites' spectral absorbance was used to detect a spectral pattern per S. grandis colony by caste. PLS-DA regression with NIRS proved to be an approach with 99.9% accuracy for identifying the colonial origin of S. grandis workers and 98.3% for soldiers. The methodology showed the importance of qualitatively characterizing the colonial phenotypic response of this species. NIRS is a high-precision approach to identifying the colony origin of S. grandis workers and soldiers. The PLS-DA can be used to design ecological field studies to identify colony territorial competition and foraging behavior of subterranean termite species.

Flight behavior is an important component to understand in the context of pest management. However, because of their small size, little is known about the flight capacity of most stored-product insects, and when a flight has been assessed, it usually consists of a propensity for initiating flight. Despite a priori expectations of the importance of flight for moths, there are no data about the flight capacity and little on the flight behavior of the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). As a result, the objective of the current study was to (i) characterize the baseline flight capacity of E. kuehniella and (ii) determine how flight capacity is affected by the presence of kairomonal, pheromonal, or no stimuli. We found adult E. kuehniella flew a mean of 24–34 km in a 24-h period, and the distance flown per bout increased from 91 to 207 m in the presence of pheromones but decreased to 41 m when food was nearby compared to a negative control. The total number of flight bouts was 1.6-fold higher in the presence of pheromone compared to the negative control, but E. kuehniella flew significantly slower with pheromone and food cues present, suggesting they may be exhibiting an optimal foraging strategy. Our data on flight capacity results in qualitatively and quantitatively different conclusions about flight than those conclusions formed if only flight initiation is considered. Overall, this novel information is useful for understanding the spread within facilities and in the landscape (between facilities), as well as parameterizing ecological modeling.

Graphical Abstract

A geometric morphometric analysis was performed on the right wing of adult Calliphora vicina (Robineau-Desvoidy) collected across 4 altitudinal levels in Sicily. The objective of this study was to assess differences in shape and centroid size (CS) between females and males and across elevations. The wings analyzed in this study were removed from adults of C. vicina collected with baited traps at 20, 700, 1,153, and 1,552; for this study, 19 landmarks were identified in each wing. The coordinates of the landmarks were aligned and superimposed to prevent variations due to position, orientation, and scale; they were then scaled to the same CS and recentered. CS and Procrustes differences were, respectively, used to assess variations in size and shape. Significant differences were observed in wing shape between males and females but not between all altitudinal levels. Female wings were found to be significantly larger than males (P < 0.01). Wings of flies collected at the highest altitudinal level resulted in significantly larger wings than those collected at lower altitudes (P < 0.001), with CS values ranging from 12.1 to 14.1. Variation in wing shape can impact thermal regulation, and therefore, oxygen content, temperature, atmospheric pressure, and solar radiation can have an effect on an insect's body and activity levels. At high elevations and lower temperatures, larger wings could mean less energy expenditure when flying to increase body temperature.

Graphical Abstract

Domestication can lead to significant changes in the growth and behavior of organisms. While the threat of predation is a strong selective force in the wild, the relaxation or removal of this threat in captive-rearing environments selects for reduced sensitivity to biotic stressors. Previous work has documented such changes in other taxa, but no work has been done on domestication-related losses of predation risk sensitivity in insects. We exposed both wild and domesticated (>50 generations in captivity) Lymantria dispar dispar (Lepidoptera: Erebidae) larvae to recordings of predators (wasp buzzing), nonpredators (mosquito buzzing), or no sound to compare the effects of predation risk on the two stocks. Wasp buzzing, but not mosquito buzzing, decreased survival of wild caterpillars relative to the control; domesticated caterpillars showed no such response. Domesticated L. dispar larvae appear to have reduced sensitivity to predation risk cues, suggesting that captive-reared insects may not always be analogs to their wild counterparts for risk-related behavioral studies.

Microplastics (MPs) and nanoplastics (NPs) are pervasive environmental pollutants that are commonly ingested by organisms at different trophic levels. While the effects of MPs on aquatic organisms have been extensively studied, the impacts of MP ingestion on the host fitness of terrestrial organisms, mainly insects, have been relatively unexplored. This study investigates the effects of MP and NP ingestion on the survivorship and reproduction of 2 medically important mosquito species, Aedes aegypti Linnaeus (Diptera: Culicidae) and Aedes albopictus Skuse (Diptera: Culicidae). Larval and pupal survivorship of Ae. albopictus were not significantly affected by particle size or concentration, but there was a reduction of Ae. aegypti pupal survivorship associated with the ingestion of 0.03 µm NPs. In addition, there was little observed impact of 0.03 µm NP and 1.0 µm MP ingestion on adult survivorship, fecundity, and longevity. To further investigate the effects of MP ingestion on mosquito fitness, we also examined the effects of MPs of varying shape, size, and plastic polymer type on Ae. aegypti immature and adult survivorship. The data suggest that the polymer type and shape did not impact Ae. aegypti immature or adult survivorship. These findings highlight that understanding the effects of microplastic ingestion by mosquitoes may be complicated by the size, composition, and amount ingested.

Chironomids of the genus Diamesa (Meigen, 1835, Diptera: Chironomidae) inhabit cold, oxygen-rich running waters. We have investigated the presence of Diamesa and other freshwater macroinvertebrates at 22 stream sampling sites in 3 European high mountain regions (the Central Pyrenees, the Ötztal Alps, and the Tatra Mountains) to establish suitable temperature conditions for Diamesa dominance. It has been generally accepted that their high abundance was linked to the presence of glaciers; however, we have shown that in the Tatra Mountains, where there are no glaciers, the conditions for the dominance of Diamesa species are created due to permanent snowfields, the geographical orientation of the valley and shading by the surrounding high peaks. The historical connection of Diamesa to glaciers was investigated from the paleolimnological records of subfossil chironomid assemblages from the Bohemian Forest, where glaciers disappeared before or during the Late Glacial period.

As expected, water temperature seems to be the main driver of Diamesa distribution, and we determined that the relative abundance of Diamesa species was significantly higher at the sites with a mean July water temperature below 6.5 °C.The Diamesa-dominated stream communities seems to be endangered due to ongoing climate warming and this assumption is supported by our paleolimnological results from the Bohemian Forest lakes, where Diamesa has disappeared due to warming of lake inflows at the beginning of the Holocene.These findings strengthen the former suggestions that some Diamesa species could be used as an indicator for tracking recent environmental changes in vulnerable ecosystems of cold mountain streams.

A study was carried out in 345 water ecosystems (rivers, streams, ditches, lagoons, ponds, puddles, rockpools, and artificial containers) throughout the autonomous community of Galicia (NW Spain) during different seasons between 2020 and 2023.The results revealed the first detections of Anopheles (Anopheles) petragnani Del Vechio, 1939 (n = 185) and Culex (Culex) mimeticus Noè, 1899 (n = 12) in the territory, allowing to update and expand the overall knowledge about their distribution and ecology. The breeding preferences of the species were analyzed considering habitat characteristics (land use, water body type, and degree of insolation), geographical variables (latitude, longitude, and altitude), and physical–chemical water parameters (temperature, pH, dissolved oxygen, electrical conductivity, salinity, total dissolved solids, and turbidity). In addition, the relationship between the occurrence of these species and other mosquitoes present in the study area was discussed. Anopheles petragnani is widely distributed in the region and shows breeding preferences for water bodies of fluvial origin, forest environments, and shaded situations. Culex mimeticus was detected sporadically breeding in a pond and in a river in the south of the region, both in industrial and agricultural land uses, and always exposed to the sun. Although the presence of these species may not currently pose a health risk in the region, it is important not neglecting their study since the adequate characterization of their larval biotopes is relevant regarding vector surveillance and control measures.

Se realizó un estudio en 345 ecosistemas acuáticos (ríos, arroyos, canales, lagunas, charcas, charcos, charcas fluviales y recipientes artificiales) a lo largo de la comunidad autónoma de Galicia (NO España) durante diferentes épocas entre 2020 y 2023. Los resultados revelaron las primeras detecciones de Anopheles (Anopheles) petragnani Del Vechio, 1939 (n = 185) y Culex (Culex) mimeticus Noè, 1899 (n = 12) en el territorio, permitiendo actualizar y ampliar el conocimiento general sobre su distribución y ecología. Se analizaron las preferencias de cría de las especies considerando las características del hábitat (uso del suelo, tipo de cuerpo de agua y grado de insolación), las variables geográficas (longitud, latitud y altitud) y los parámetros físico-químicos del agua (temperatura, pH, conductividad eléctrica, salinidad, total de sólidos en suspensión y turbidez). Además, también se analizó la relación entre la ocurrencia de estas especies y otros mosquitos presentes en el área de estudio. Anopheles petragnani está ampliamente distribuido en el territorio y muestra preferencia por aguas de origen fluvial, ambientes forestales y zonas sombreadas. Culex mimeticus se detectó puntualmente criando en una charca y un río en el sur de la región, tanto en ambientes industriales como agrícolas, y siempre en zonas soleadas. Aunque actualmente la presencia de estas especies no supone un riesgo sanitario en la región, es importante no descuidar su estudio ya que la adecuada caracterización de sus biotopos larvarios es relevante en términos de vigilancia y control vectorial.

Ensuring the safety of insecticides to natural enemy insects of pests is crucial for integrating chemical and biological control strategies. Broflanilide, a novel meta-diamide insecticide, exhibits high insecticidal activity against Myzus persicae (Sulzer) (Hemiptera: Aphididae). To integrate chemical and biological control against M. persicae, we assessed the toxicity of broflanilide to Aphidius gifuensis, and evaluated its safety and sublethal effects. The LC₁₀, LC₂₅, and LC₅₀ values of broflanilide against A. gifuensis were 0.733 mg/L, 1.613 mg/L, and 3.852 mg/L, respectively. The selectivity toxicity ratio of broflanilide to A. gifuensis was 1.516, indicating higher toxicity to M. persicae compared to A. gifuensis. The risk quotient of broflanilide to A. gifuensis adults was 6.18. The percent reduction in the emergence of the parasitoid pupae was –1.15, with a risk grade of 1. The sublethal concentration of broflanilide had no significant influence on the intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (R₀), and mean fecundity (F) of A. gifuensis in the F₁ generation. The mean generation time (T) increased by 0.51 days and 0.39 days in the LC₁₀ and LC₂₅ treatments, respectively; the difference between LC₁₀ treatment and the control was significant, while the difference between LC₂₅ treatment and the control was not significant. The results showed that the sublethal concentration of broflanilide did not have a significant inhibitory effect on the population growth of A. gifuensis.

Graphical Abstract

Coffee berry borer (Hypothenemus hampei Ferrari) (Coleoptera: Curculionidae) is the most damaging insect pest of coffee worldwide, causing significant losses in coffee yields and quality. Knowledge of vertical and temporal flight patterns in coffee berry borer could be used to optimize spray timing and precision targeting of areas within the coffee tree, which may be more susceptible. In the present study, we estimated the vertical distribution of coffee berry borer females using traps set at 1-m intervals up to 5 m in height. We also quantified coffee berry borer infestation in the low, mid, and high canopy and documented fruit availability. Temporal flight patterns were estimated using timer traps, and correlation analyses were conducted to determine the relationship between the timing of daily flight and weather variables. Across the 4 study sites, we observed that 77%–84% of the trap catch was at 1 m, 11%–20% was at 2 m, and 1%–4% was at 3–5 m in height. Fruit infestation was significantly higher in the low branches (35%) relative to the high branches (17%). Flight height remained the same year-round, regardless of fruit availability. Coffee berry borer flew in low numbers during the day and night but peaked from 12 to 4 PM. Daily flight was positively correlated with an increase in air temperature and wind speed and negatively correlated with relative humidity. Findings from this study suggest that pesticide sprays should target low- to mid-level branches at 1–2 m in height and aim to be conducted in the early afternoon when coffee berry borer are actively flying and most vulnerable to chemical controls.

There are widespread public efforts to conserve wildlife in urbanized landscapes via the installation of nursery-grown plants that support Lepidoptera taxa. Insecticides are commonly used during nursery production to suppress key plant pests, and many products have extended periods of toxicity and affect a wide range of herbivore taxa. While there are plentiful toxicological data on bee species, predominantly the Western honey bee (Apis mellifera L.), little is known about how insecticides affect nonpest lepidopterans. Lepidoptera has different modes of exposure (e.g., leaf-feeding) and differences in susceptibility to insecticide target sites compared to bees. Consequently, many products compatible with bee conservation pose an uncertain risk to nonpest lepidopterans and thus may represent an under-recognized conflict with conservation efforts. Using the monarch butterfly (Danaus plexippus, L.), tropical milkweed (Asclepias curassavica, L.), and oleander aphid (Aphis nerii, Fonscolombe, 1841) system, we conducted leaf and whole-plant feeding assays to evaluate effects of acute and chronic monarch exposure to industry standard and alternative reduced-risk insecticides used during nursery production. We also evaluated the efficacy of these insecticides against their target pest, the oleander aphid. Our results indicate that insecticides used to control pests on ornamental milkweed can cause monarch larval mortality up to 4 wk after treatment application. Furthermore, the duration of aphid suppression is often shorter than the duration of adverse effects on monarchs. This study demonstrates a conflict between insect pest management and Lepidoptera conservation during ornamental plant production and has implications for the conservation value of ornamentals after retail sale.

The effect of the 20th-century functional extinction of the American Chestnut (Fagaceae: Castanea dentata (Marshall) Borkh) on associated herbivorous insects is unknown. These insects include leafminers that spend at least part of their larval phase feeding between the epidermises of leaves. We surveyed leafminers on C. dentata, nonnative Castanea spp., and hybrids on Long Island, NY. We found 10 leafminer species feeding on Castanea spp. A first New York State record was documented for Stigmella castaneaefoliella (Chambers) (Lepidoptera: Nepticulidae). New host records are established for 6 lepidopterans, including a new host genus for Phyllonorycter basistrigella (Clemens) (Lepidoptera: Gracillariidae). We found no significant differences in the mean intensity of S. castaneaefoliella leaf mines on native and nonnative Castanea spp.; however, our sample size was small. Thus, we guardedly conclude that nonnative Castanea spp. can serve as refugia for C. dentata leafminers native to North America while acknowledging that the extent to which nonnative species are utilized requires further investigation.

The evolution of oviposition preference in insects is considered a key evolutionary strategy in the context of host–plant interaction. It is hypothesized that insects maximize the survival and fitness of the subsequent generations by preferring specific host plant(s), known as the “preference-performance hypothesis.” In this study, we tested whether adult host preference reflects the immature performance in an oligophagous insect, Phthorimaea absoluta Meyrick, a rapidly emerging invasive pest in Asia, Africa, and Europe. Based on a preliminary survey of the potential host plants of P. absoluta, we selected 6 Solanaceae species, namely, tomato, potato, eggplant, black nightshade, sweet pepper, and tobacco, for the oviposition preference studies. The results indicated that the tomato was the most preferred host in no-, dual- and multiple-choice assays, followed by potato, eggplant, and black nightshade. Subsequently, the insect life-table parameters were found to be superior on tomato compared to other hosts. The order of oviposition preference on the host plants was strongly correlated with the life-table parameters of P. absoluta. Thus, we provide clear evidence for the preference–performance hypothesis in the host selection behavior of P. absoluta. We also emphasize the necessity of conducting oviposition behavior research at various geographic locations to develop tailor-made integrated pest management programs.

Pear psylla, Cacopsylla pyricola (Foerster) (Hemiptera: Psyllidae), occurs as 2 seasonal morphotypes. Summerforms occur on pear (Pyrus communis L.; Rosales: Rosaceae) where they are a significant pest. The larger and darker winterform morphotype develops in response to shortening daylengths and begins winter in reproductive diapause characterized by the absence of ovarian development. Diapausing winterforms often leave pear to overwinter on coniferous shelter plants and then return to pear in late winter and early spring to begin depositing the eggs that produce the first summerform generation. Cacopsylla pyricola adults are attracted to the color of foliage most of the year, but little is known about the role of plant volatiles in host finding and in seasonal dispersal between host and shelter plants by the psyllid. We used a Y-tube olfactometer and choice assays to investigate the response by C. pyricola adults to volatiles emitted by pear and an evergreen tree (cypress) often used as a shelter plant by wintering C. pyricola. Attraction to pear and cypress volatiles varied by season, tree phenology, and psyllid physiology. Cacopsylla pyricola were attracted to cypress volatiles and preferred to settle on cypress shoots during winter and early spring but then shifted to a marked preference for the pear developmental host in late spring and summer. Female C. pyricola exhibited stronger responses to pear volatiles than did males. Our study is the first to show that plant volatiles have a role in host finding by C. pyricola and provides a foundation for research on chemical ecology and management of C. pyricola.

The life history aspects of dormancy of the weevil Anthonomus rufipennis LeConte (Coleoptera: Curculionidae) were studied a 57-month period in a seasonally dry tropical forest of central Mexico. Weevil populations and their physiological status were monitored on both the reproductive host tree, Senna polyantha (Collad.) H.S: Irwin & Barneby (Fabales: Fabaceae) and the highly favored refuge host, Tillandsia recurvata L. (Poales: Bro meliaceae) or “ball moss.” During the dry season, weevils were only found on the refuge host with a mean total density of 1.014 ± 2.532 individuals/ball moss (N = 1,681). Weevil densities on T. recurvata between early and late dry seasons were not significantly different, suggesting that dry season survival was relatively high. Weevils collected during these seasons revealed little reproductive development and relatively high-fat accumulation in both sexes. During 5 of 6 yr, densities of the weevils in T. recurvata dropped significantly during the early rainy seasons, when the reproductive host trees leafed out and began producing oviposition sites (flower buds). At this time, more males than females initially moved to vegetative trees and showed significant signs of reproductive development. Recolonization of ball moss by weevils began during the late rainy season when oviposition sites (flower buds) were still available. A proportion of the weevils remained on the reproductive host, suggesting that A. rufipennis is facultatively multivoltine.The methodologies and results of the study can serve as a model system for future studies of the dormancy of other insects in dry tropical forests and provide insight into the dormancy of other anthonomine weevils of economic importance.

Apis mellifera Linnaeus (Hymenoptera: Apis), honey bees, are the most widely used managed crop pollinators. However, their high rental cost and uncertain availability for North American orchard crops have motivated growers to explore alternative pollination options. We examined whether adding solitary, spring-flying Osmia lignaria Say (Hymenoptera: Megachilidae), blue orchard bees, as co-pollinators with A. mellifera in Washington sweet cherry and pear orchards enhances fruit set and yield compared to the use of A. mellifera alone. We added managed O. lignaria to orchard sites where A. mellifera hives were already present. Fruit set, fruit yield, and O. lignaria reproduction at O. lignaria-supplemented sites were compared to nearby, paired sites pollinated only by A. mellifera (3 paired cherry and 3 paired pear sites). For both crops, the addition of O. lignaria significantly increased fruit set but did not yield at harvest. Microscopic inspection of pollen grains from O. lignaria nest cell provisions confirmed that O. lignaria primarily visited orchard flowers. Mean retention of O. lignaria in cherry orchards was slightly higher (65%) than O. lignaria retention reported in other orchard crops (30%–60%). However, retention in pear orchards was much lower (≤20%). These results show that supplementing hives with O. lignaria in Washington spring orchard crops can increase overall pollination, but that trees fail to bear developing fruit to maturity. The strategy of using co-pollinators, O. lignaria and A. mellifera, in US orchards may act as “pollination insurance” when A. mellifera hives are in low supply or when the weather is not amenable for A. mellifera flight during the bloom period.

Bumble bees are declining across the globe. The causes of this decline have been attributed to a variety of stressors, including pesticides. Fungicides are a type of pesticide that has been understudied in the context of bumble bee health. As a result, fungicides are often applied to flowering plants without consideration of pollinator exposure. Recent work demonstrates that fungicides have sublethal effects in bumble bees, but little is known about how much fungicide it takes to cause these sublethal effects. To address this gap in the literature, we fed microcolonies of the common eastern bumble bee (Bombus impatiens CressonHymenoptera: ApidaeHymenoptera: ApidaeHymenoptera: ApidaeHymenoptera: Apidae) pollen contaminated with a range of fungicide concentrations. We chose these concentrations based on the range of fungicide concentrations in pollen and nectar that were reported in the literature. Results revealed that later-stage pupae and newly emerged males are potentially sensitive to fungicide exposure, showing smaller size and reduced fat reserves at intermediate levels of contamination. Compared to the control, intermediated levels of fungicide-contaminated pollen led to increased pupal mortality and delayed male emergence. Contrary to expectations, higher fungicide levels did not exhibit a linear relationship with negative impacts, suggesting nuanced effects. Because body size and emergence timing are important aspects of bumble bee reproductive behavior, results have implications for mating success, potentially disrupting colony development.

Neonicotinoid insecticide seed treatments are commonly used in rice (Oryza sativa) production to control rice water weevil (Lisorhoptrus oryzophilus). With the use of neonicotinoid seed treatments, there is potential that honey bees (Apis mellifera) could be exposed to neonicotinoids through translocation to the pollen. Studies were conducted in 2015 and 2016 to determine the level of neonicotinoids present in flag leaves, pollen, and grain of rice. Thiamethoxam was applied as a seed treatment and foliar prior to flooding. Clothianidin was applied as a seed treatment and as a foliar at a preflood and postflood timing. Subsamples of flag leaves, pollen, and grain were analyzed for positive neonicotinoid detections and abundance. Thiamethoxam was detected in 8.9% of samples and clothianidin was detected in 1.4% of samples. For both thiamethoxam and clothianidin, more positive samples were observed in flag leaf samples than in pollen or grain. An average of 4.30 ng/g of thiamethoxam was detected in flag leaves from seed-applied thiamethoxam. An average of 1.25 ng/g of clothianidin was found in flag leaves from a preflood application of clothianidin. A survey of honey bees present in rice fields was conducted in Mississippi and Arkansas to determine the abundance of honey bees present in rice fields based on the time of day. Honey bee densities were low in rice, with less than 5% and 3% positive detections observed in Mississippi and Arkansas, respectively. More positive detections and higher densities of honey bees were observed for mid-day sampling than for morning or evening sampling.

The leaf-mining microlepidopteran, Macrosaccus morrisella (Fitch) (Lepidoptera: Gracillariidae), has emerged as a new pest of soybean, Glycine max (L.) Merrill (Fabales: Fabaceae), in Canada and the United States, but little is known about its life history traits. Thus, this study was conducted to assess the immature developmental rate of M. morrisella, from egg to adult emergence, on soybean at different temperatures, and the longevity of adults supplied with water and/or honey at different temperatures.The time to 50% emergence of adults was 71.90, 36.33, 24.62, and 17.83 days at 15, 20, 25, and 30 °C, respectively.The lower developmental threshold of M. morrisella was estimated at 8.96 °C, with 425.04 degree-days required for egg-to-adult development. For adult longevity, time to 50% mortality at 25 °C was 15.00, 4.00, and 2.00 days when adults were provided with 25% (v/v) honey–water solution, water, or nothing, respectively. In a follow-up experiment, time to 50% mortality at 25 °C was 24.00, 6.00, 3.00, and 3.00 days when adults were provided with honey and water (offered separately), honey, water, or nothing, respectively, with a synergistic effect when honey and water were offered simultaneously as opposed to honey or water alone. Finally, when fed 25% (v/v) honey–water solution and maintained at 20, 25, and 30 °C, time to 50% mortality of adults was 26.50, 15.00, and 15.00 days. These results inform the understanding of the basic biology of M. morrisella and will help inform the future development of management programs for this insect in soybean.

The All Taxa Biodiversity Inventory (ATBI) in Great Smoky Mountains National Park (GSMNP) seeks to document every species of living thing in the park. The ATBI is decades in progress, yet some taxa remain virtually untouched by taxonomists. Such “high priority” taxa include the hyper-diverse parasitoid wasp family Ichneumonidae. Despite the positive and multifaceted effects ichneumonids have on their environment, only a small percentage of those collected in the park have been identified as species, mostly to their complex morphology and overwhelming diversity. Recently, DNA barcoding has transformed biodiversity inventories, streamlining the process to be more rapid and efficient. To test the effectiveness of barcoding 20 + year-old specimens of Ichneumonidae and catalog new records for GSMNP, COI was amplified from 95 ichneumonid morphospecies collected from Andrew's Bald, NC. Species identifications were confirmed morphologically. Eighty-one ichneumonids generated sequence data, representing 16 subfamilies and 44 genera.The subfamily Oxytorinae is newly recorded from GSMNP, along with 10 newly recorded genera and 23 newly recorded species across Ichneumonidae. These results contribute significantly to the ATBI by adding new park records for a high-priority taxon and demonstrate the effectiveness of applying DNA barcoding to samples in long-term storage or those lacking immediate taxonomic expertise.

The twisted-wing parasite order (Strepsiptera Kirby, 1813) is difficult to study due to the complexity of strepsipteran life histories, small body sizes, and a lack of accessible distribution data for most species. Here, we present a review of the strepsipteran species known from NewYork State. We also demonstrate successful collection methods and a survey of species carried out in an old-growth deciduous forest dominated by native NewYork species (Black Rock Forest, Cornwall, NY) and a private site in the Catskill Mountains (Shandaken, NY). Additionally, we model suitable habitats for Strepsiptera in the United States with species distribution modeling. We base our models on host distribution and climatic variables to inform predictions of where these twisted-wing parasites are likely to be found.This work provides a useful reference for the future collection of Strepsiptera.