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Effective management of the introduced invasive grass common reed [Phragmites australis (Cav.) Trin. ex Steud.] requires the ability to differentiate between the introduced and native subspecies found in North America. While genetic tools are useful for discriminating between the subspecies, morphological identification is a useful complementary approach that is low to zero cost and does not require specialized equipment or technical expertise. The objective of our study was to identify the best morphological traits for rapid and simple identification of native and introduced P. australis. A suite of 22 morphological traits were measured in 21 introduced and 27 native P. australis populations identified by genetic barcoding across southern Ontario, Canada. Traits were compared between the subspecies to identify measurements that offered reliable, diagnostic separation. Overall, 21 of the 22 traits differed between the subspecies, with four offering complete separation: the retention of leaf sheaths on dead stems; a categorical assessment of stem color; the base height of the ligule, excluding the hairy fringe; and a combined measurement of leaf length and lower glume length. Additionally, round fungal spots on the stem occurred only on the native subspecies and never on the sampled introduced populations. The high degree of variation observed in traits within and between the subspecies cautions against a “common wisdom” approach to identification or automatic interpretation of intermediate traits as indicative of aberrant populations or hybridization. As an alternative, we have compiled the five best traits into a checklist of simple and reliable measurements to identify native and introduced P. australis. This guide will be most applicable for samples collected in the late summer and fall in the Great Lakes region but can also inform best practices for morphological identification in other regions as well.
Greenhouse experiments were conducted in 2020 to investigate the effects of carrier volume and sethoxydim rate on torpedograss (Panicum repens L.) control and sand cordgrass (Spartina bakeri Merr.) response from a single application. Panicum repens control and biomass reduction generally increased with increasing sethoxydim rates in evaluations at 14, 28, and 42 d after treatment (DAT); however, increasing the rate to 2X the maximum labeled rate did not always result in increased efficacy. In the first experimental run, which consisted of small plants, P. repens control and biomass reductions were largely similar among tested carrier volumes (37, 187, and 935 L ha–1). However, in run 2, which consisted of larger, mature P. repens plants, efficacy increased when carrier volume was reduced. Spartina bakeri injury increased with sethoxydim rate, reaching a maximum of 45% by 42 DAT. However, no differences in S. bakeri injury among carrier volumes were observed at 14 and 28 DAT evaluations. Spartina bakeri aboveground biomass reductions were also largely driven by sethoxydim rate increases rather than reduced carrier volumes, reaching 40% to 50% reduction in initial aboveground biomass. However, S. bakeri belowground biomass was 20% to 32% greater in treatments applied at 37 or 187 L ha–1 compared with those at 935 L ha–1. Overall, these data suggest that selective P. repens control with sethoxydim may be enhanced through reducing carrier volumes from 935 L ha–1 and that native, perennial, caespitose grasses may exhibit greater tolerance to sethoxydim compared with the rhizomatous P. repens. Future research should further test these hypotheses under field conditions at operational scales.
Japanese knotweed (Reynoutria japonica Houtt.) is an invasive Asian plant abundant along rivers in its introduced range. In riparian areas, floods and ice flows uproot the rhizomes, facilitating their dissemination downstream. Control of large, well-established R. japonica clones in riparian areas is difficult if the use of herbicides is prohibited. An alternative to controlling entrenched clones is the rapid detection and manual unearthing of rhizome fragments that have recently rooted after being deposited by floodwaters. We applied this strategy along a Canadian river where spring floods with abundant ice are recurrent. Two river stretches, with approximately 10 km of shoreline each, were selected for the fragment removal campaign. One of the stretches was heavily invaded by R. japonica, while the other was only sparsely invaded. In the heavily invaded stretch, 1,550 and 737 R. japonica rhizome fragments were unearthed in 2019 and 2020, respectively. Unearthed fragments had an average length of 27 to 32 cm. Only 21 fragments were found in the sparsely invaded stretch in 2020. Despite similar distances being surveyed, the detection and unearthing took 62% less time (overall) in the sparsely invaded than in the heavily invaded stretch. Along sparsely invaded riverbanks, a rapid response removal campaign for R. japonica cost, including transportation and labor, an estimated Can$142 (US$105) per aborted clone (i.e., fragment removed). A rapid response removal campaign is economically advantageous compared with the hypothetical eradication of large, well-established clones, but for it to be cost-effective, the time spent locating rhizome fragments must exceed the time spent unearthing them. The question is not whether rapid response unearthing is economically feasible—it is—but rather what invasion level renders the intervention practicable. In highly invaded river stretches generating thousands of fragments annually, finding and removing these fragments year after year would require a massive, unsustainable effort.
Expanding the current aquatic herbicide portfolio, reducing total spray volumes, or remotely delivering herbicide using novel spray technologies could improve management opportunities targeting invasive aquatic plants, where options are more limited. However, research on giant salvinia (Salvinia molesta Mitchell) response to foliar herbicide applications at carrier volumes ≤140 L ha–1 is incomplete. Likewise, no data exist documenting S. molesta control with unoccupied aerial application systems (UAAS). Following the recent >100-ha incursion of S. molesta in Gapway Swamp, NC, a case study was developed to provide guidance for ongoing management efforts. In total, three field trials evaluated registered aquatic and experimental herbicides using a 140 L ha–1 carrier volume. Select foliar applications from UAAS were also evaluated. Results at 8 wk after treatment (WAT) indicated the experimental protoporphyrinogen oxidase inhibitor, PPO-699-01 (424 g ai ha–1), in combination with endothall dipotassium salt (2,370 g ae ha–1) provided 78% visual control, whereas control when PPO-699-01 (212 g ai ha–1) was applied alone was lower at 35%. Evaluations also showed diquat (3,136 g ai ha–1) alone, glyphosate (4,539 g ae ha–1) alone, and metsulfuron-methyl (42 g ai ha–1) alone achieved 86% to 94% visual plant control at 8 WAT. Sequential foliar applications of diquat, flumioxazin (210 g ai ha–1), and carfentrazone (67 g ai ha–1) at 6 wk following exposure to in-water fluridone treatments were no longer efficacious by 6 WAT due to plant regrowth. Carfentrazone applications made from a backpack sprayer displayed greater control than applications made with UAAS deploying identical carrier volumes at 2 WAT; however, neither application method provided effective control at 8 WAT. Additional field validation is needed to further guide management direction of S. molesta control using low carrier volume foliar applications.
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