The effects of mating status, sex, beetle age, host quality, temperature, and wind speed on the propensity of Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae) to take flight were evaluated using a free flight test in the laboratory.Time to initiate flight, the angle of flight, and flight capability (when beetles were enticed to take flight) were also evaluated. Host quality, mating status, beetle age, sex, temperature, and the interactions between one or more of these were all found to be significant predictors of flight in A. glabripennis in one or more of the experiments. Female flight propensity peaked at sexual maturity and declined thereafter. Both sexes had a higher propensity to take flight from a stem section of dry host material than from a fresh one. Most (78%) males flew at least once during the four mating status/ages tested from a fresh host stem section, while only 43% of the females flew at least once after chewing an oviposition pit. Flight propensity and distance flown increased with temperature and there was no voluntary flight at 15°C. Flight propensity did not increase with wind speeds 0.0–1.0 m/s, but no ascending flight was observed at 0.5 or 1.0 m/s. Time to flight initiation did not vary with the factors evaluated. Implications these results could have on the success of eradication programs are discussed. Specifically, what factors increase the propensity of mated females to disperse, effectively expanding the infestation zone.