Scrobipalpa ocellatella (Boyd) (Lepidoptera: Gelechiidae) is one of the most important pests of sugar beet that causes quantitative and qualitative yield loss in the late summer. To locate the position for diapause induction, combinations of constant temperatures at 15, 18, 20, and 25°C and day lengths of 8, 10, 12, 14, 16, and 24 h were studied from egg to adult emergence. The incidence of diapause peaked at 15 and 18°C, with the day lengths of 12 and 11 h, whereas low temperatures did not improve the effects of short photoperiods (day lengths of 8 and 10 h) in diapause induction. The results showed that the critical day length for diapause induction was 12.8 h at overall 15 and 18°C. It was observed that the third instar larvae were the most sensitive stage to the inductive photoperiod (12:12 [L:D] h). The non-24-h light-dark experiment showed that the nigh length is more important than the day lengths measurement. In a set of 24-h light-dark cycles at 2:12 (L:D) h, a 1-h light pulse declined diapause induction markedly 1 h after scotophase. Field monitoring of the S. ocellatella for 2 yr (2015 and 2016) showed that the 50% of larvae enter winter pupal diapause in early September and this proportion increases in response to a decrease in the day lengths and temperature. From this study, it was concluded that low temperature acts in conjunction with short-day photoperiod in diapause induction of S. ocellatella.