Masood Ghasemi Ghehsareh, Sayyed Mohammad Ehsan Mahdavi, Abbas Peyrovan, Mehdi Ghasemi Nafchi, Bruce L. Dunn
Canadian Journal of Plant Science 104 (1), 21-31, (8 September 2023) https://doi.org/10.1139/cjps-2023-0039
KEYWORDS: geophytes, plant propagation, Plant growth regulators, fall-blooming flower, climate change
Sternbergia clusiana (SC) is a bulbous plant having an alluring ornamental flower, medicinal properties, and an endangered status due to climate-change ecology. However, the methods of propagation for conserving this notable species have been neglected. The main aim of this study was to evaluate the influence of plant growth regulators (PGRs) on SC regeneration by virtue of a asexual propagation method, with the intention of preserving this endangered species from extinction. In this regard, having collected the bulbs from a wild habitat, bulbs were cut in accordance with the chipping technique. Thereafter, pieces were treated with 6-benzylaminopurine (BAP) at 250, 500, and 1000 mg·L−1, 1-naphthaleneacetic acid (NAA) at 100, 250, and 500 mg·L−1, putrescine (PU) at 50, 100, and 200 mg·L−1, BAP and NAA combination, BAP and PU combination, and a control. Pieces were then transferred into primary and terminal growing media under laboratory and greenhouse conditions, respectively. Next, the number, length, width, and weight of bulbs were determined. The greatest bulb number (2.10 counts), bulb length (26.75 mm), bulb width (16.25 mm), and bulb weight (3.13 g) were observed in the PU treatments. In addition, the results showed that even though the treated bulbs with BAP and PU combinations had preferable performance generally, the results were inconsistent. Taken together, this research indicates that utilizing asexual propagation along with PGRs, in the proper dosage, can serve as a mechanism to protect the endangered geophyte from extinction in nature.