There is considerable agreement that the gastrointestinal microbiota contributes to the performance and health of the neonate, and this relationship includes an ability of the host animal to “sense” changes in the microbial community. Identifying the mechanisms used by the host to sense microbiota is one approach to developing methods to manipulate the microbiota to improve pig health and performance. Diet-independent microbial products are molecules unique to the microbial community and sensed by host pattern recognition receptors stimulating inflammation. Common among all members of the microbial community, their presence is unaffected by diet, but the nature of the response does depends on factors affecting the microenvironment in which the molecule is detected. Diet-dependent microbial products arise as products of fermentation of dietary components and include short-chain fatty acids, ammonia, phenols, hydrogen sulfide, amines, and many other compounds. A plethora of sensing mechanisms exists that include enzymatic metabolism as well as membrane receptors that have evolved to respond to microbial products (e.g., short-chain fatty acid receptors), or simply cross-react with microbial products. This review focuses on host mechanisms used to sense the intestinal microbiota and attempts to establish practical considerations for neonatal gut health based on current understanding.

Legume forages, such as sainfoin (Onobrychis viciifolia Scop.) and birdsfoot trefoil (Lotus corniculatus L.), can increase the forage quality and quantity of Western Canadian pastures, thus increasing producer profitability due to increased gains in grazing ruminants, while also reducing risk of bloat in legume pastures due to the presence of proanthocyanidins. Proanthocyanidins or condensed tannins (CT) are secondary plant polyphenol compounds that have been regarded as anti-nutritional due to their ability to bind protein in feeds, enzymes, and microbial cells, therefore disrupting microbial digestion and slowing ruminal protein and dry matter (DM) digestion. Research has shown that at high concentrations (>50 g·kg⁻¹ DM), CT can disrupt microbial digestion. However, at low dietary inclusion rates (5–10 g·kg⁻¹ DM), they reduce bloat risk, increase ruminal undegradable protein, reduce enteric methane production, and benefit anthelmintic activity. Yet, research gaps still exist regarding grazing persistence and forage yield of novel CT-containing forages and their biological activity due to their vast differences in CT stereochemistry, polymer size, and intermolecular linkages. The objectives of this review are to summarize information regarding the impact of CT on ruminal fermentation, carbohydrate and protein metabolism, and the potential to identify and select for forages that contain CT for ruminant production.

An experiment was conducted to determine the standardized ileal digestibility (SID) of amino acids (AA) in distillers dried grains with solubles derived from corn (C-DDGS), sorghum (S-DDGS), and triticale (T-DDGS) fed to growing pigs. A total of 16 barrows [initial body weight (BW) = 23.1 ± 2.03 kg] surgically fitted with T-cannulas at the distal ileum were divided into four groups based on BW and assigned to a quadruplicated 4 × 2 incomplete Latin square design with four dietary treatments and two experimental periods. Three diets containing C-DDGS, S-DDGS, or T-DDGS, as well as a nitrogen-free diet, were fed in each experimental period consisting of 5 d of adaptation and 2 d of ileal digesta collection. Pigs fed S-DDGS had less (P < 0.05) SID of histidine, isoleucine, leucine, and methionine compared with those fed T-DDGS, which were less (P < 0.05) than the values observed for pigs fed C-DDGS. The SID of arginine, phenylalanine, threonine, and valine in C-DDGS were greater (P < 0.05) than in S-DDGS but were not different from those in T-DDGS. The SID of lysine in C-DDGS was greater (P < 0.05) than in S-DDGS and T-DDGS. In conclusion, AA in S-DDGS were less digestible than those in C-DDGS and T-DDGS.

The objectives of this study were to examine the effects of feeding newly developed blend-pelleted carinata meal (BPPCR) and blend-pelleted canola meal (BPPCN) on nutrient intake, digestibility, and production performance of high producing dairy cows. In this study, nine mid-lactating Holstein cows (body weight, 679 ± 124 kg; days in milk, 96 ± 22) were used in a triplicated 3 × 3 Latin square study. Within each square, each cow was randomly assigned to one of three treatments: control (typical barley-based diet in Western Canada), BPPCR (soybean and canola meal in the control diet replaced by BPPCR), and BPPCN (soybean and canola meal in the control diet replaced by BPPCN). Each period lasted for 21 d, with the first 14 d as an adaptation period. The total collection of feces and urine were conducted on six cows from two Latin squares. The results showed that there were no treatment effects on milk yield, milk composition, and nutrient intake and digestibility (P > 0.10). The nitrogen balance among the three treatment groups was not different either. In conclusion, replacing soybean meal and canola meal with blend-pelleted co-products did not affect the nutrient intake, digestibility, and production performance in high producing dairy cows.

The objective of this study was to estimate economic losses associated with milk performance detriments under different climate scenarios. A dataset containing milk records of Holstein and daily temperature–humidity indexes compiled over 6 yr in two contrasting climatic dairy regions of Quebec [eastern (EQ) and southwestern Quebec (SWQ)] was used to develop equations for modeling milk performance. Milk performance, including milk, fat, protein, and lactose yields of dairy herds (kg·d⁻¹), were then projected considering six plausible climate scenarios during a climatic reference period (REF: 1971–2000) and two future periods (FUT1: 2020–2049; FUT2: 2050–2079). Economic losses were assessed by comparing future to reference milk prices based on components. Results indicated that fat and protein yields could decline in the future, thus resulting in economic losses ranging from $5.34 to $7.07 CAD·hL⁻¹ in EQ and from $5.03 to $6.99 CAD·hL⁻¹ in SWQ, depending on the amplitude of future temperature and humidity changes and on whether it is milk quota or cow number which is adjusted to avoid under-quota production. The projected increase in occurrence and duration of heat stress episodes under climate change could result in substantial financial harm for producers, thereby reinforcing the necessity of implementing heat-abatement strategies on dairy farms.

Forty-eight (37.7 ± 3.4 kg, initial shrunk live weight) lambs were used in a 61 d experiment to evaluate the energy value of grease trap waste (GT) at four levels of supplementation (0%, 2%, 4%, and 6%). Supplemental GT replaced cracked corn in the basal diet. The GT contained 6.4% moisture, 3.1% impurities, and 79.8% total fatty acids (FA). Increasing GT level in diets did not affect dry matter intake and daily weight gain but linearly increased gain efficiency and estimated dietary net energy (NE). However, the ratio of observed-to-expected diet NE decreased with increased levels of GT. The estimated NE values for GT based on FA intake were in close agreement (98% and 102% of predicted, respectively) with those NE values determined by replacement technique for 2% and 4% supplementation level. However, the observed NE value for GT supplemented at the 6% level was 9% lower than predicted. Kidney–pelvic–heart fat increased as level of GT supplementation increased; otherwise, carcass characteristics and shoulder composition were not affected. We conclude that GT is a suitable alternative to conventional feed fats in diets for finishing lambs. The estimated NE of GT is 93% the energy value assigned by current standards for tallow and yellow grease.

One-day-old broiler chicks were used to investigate the optimal age to start feeding phosphorus (P)-deficient (PD) diets with two non-phytate P (nPP) concentrations in experiment 1, and 7-d-old broiler chicks were used to determine P equivalency of a hybrid 6-phytase to inorganic P in monocalcium phosphate (MCP) in experiment 2. In experiment 1, six treatments were prepared with 5, 6, or 7-d-old birds to start feeding PD diets with 1.3 or 1.5 g·kg⁻¹ nPP. Age of birds to start feeding P-deficient diets tended to decrease (P = 0.052) mortality of birds during 14 d of feeding P-deficient diets. In experiment 2, twelve treatments including one basal diet, five diets supplied with 0.5, 0.9, 1.3, 1.7, or 2.1 g·kg⁻¹ inorganic P, and six diets supplied with 250, 500, 750, 1000, 1500, or 2000 FTU·kg⁻¹ phytase were fed to chickens from days 7 to 21 post hatching. Increasing phytase activity quadratically increased (P < 0.01) body weight gain, feed intake, and tibia ash of birds and apparent ileal digestibility and total tract retention of P. In conclusion, age of birds to start feeding PD diet affected their performance, and hybrid 6-phytase may partly replace MCP in a PD diet for broiler chickens.

Six non-lactating Holstein dairy cows received diets with forage to grain mixture ratios of 100:0 (G0), 75:25 (G25), and 50:50 (G50) that contained 0.5%, 10.0%, and 19.5% dry matter (DM) of starch, respectively. Rumen fluid and feces were sampled, and methane emissions were determined during the last week of 5 wk experimental periods. Taxonomic compositions of microbiota were determined using Illumina 16S rRNA sequencing. Increased grain feeding increased the acidity and volatile fatty acid concentrations of rumen fluid and feces, and decreased methane emissions expressed as L·kg⁻¹ DM. Microbial diversities were highest for G25. The numbers of identified genera in rumen fluid were 185, 182, and 171 for G0, G25, and G50, respectively. In feces, these numbers were 197, 182, and 171 for these diets, respectively. In rumen digesta, seven genera were correlated positively to G0 and negatively to G50, and six genera were correlated negatively to G0 and positively to G50. In feces, 16 genera were correlated positively to G0 and negatively to G50, and 13 genera were correlated negatively to G0 and positively to G50. Increasing grain feeding affected a limited number of genera in rumen digesta and feces. This could not explain treatment effects on the functionalities of microbiota.

This study evaluated the efficacy of epidermal growth factor (EGF) in piglets challenged with enterotoxigenic Escherichia coli K88 (ETEC). A total of 28 piglets were assigned to the following dietary treatments for 14 d: negative control (NC) (basal diet containing supernatant without EGF), PC (NC + 2.5 g antibiotic·kg⁻¹ feed), EGF120 [basal diet + supernatant with 120 μg EGF·kg⁻¹ body weight (BW)·d⁻¹], and EGF180 (basal diet + supernatant with 180 μg EGF·kg⁻¹ BW·d⁻¹). After a 6 d acclimation period, each pig was gavaged with 6 mL (2.4 × 10¹³ cfu·mL⁻¹) of ETEC on the morning of day 7. Overall, piglets fed the EGF and PC diets tended to have higher gain to feed ratio than those fed the NC diet (P = 0.063). Pigs fed EGF diets had lower rectal temperature than those fed the NC diet at 6 h after challenge (P < 0.05). Serum and ileal malondialdehyde concentrations were higher in piglets fed the NC diet compared with those fed EGF and PC diets on days 6 and 7 after challenge, respectively (P < 0.05). In conclusion, EGF has the potential to reduce oxidative stress and body temperature elevation in piglets exposed to ETEC while supporting better feed efficiency.

A 10 wk experimental trial was carried out to determine the effect of dietary inclusion of Achyranthes japonica extract (AJE) on finishing pigs’ overall performance. A total of 150 finishing pigs with initial body weight (BW) of 54.17 ± 2.27 kg were randomly allocated to five dietary treatments. The dietary treatments were CON (basal diet), TRT1 (basal diet + 0.025% AJE), TRT2 (basal diet + 0.05% AJE), TRT3 (basal diet + 0.1% AJE), and TRT4 (basal diet + 0.2% AJE). Dietary AJE supplementation linearly increased BW, average daily gain (ADG), and gain to feed ratio (G/F) during week 5. At week 10 and overall experiment, there was tendency for linear increase in BW, ADG, and average daily feed intake. Dietary supplementation of AJE failed to show significant effects on nutrient digestibility, fecal microbiota, meat quality, and fecal gas emission. The dietary supplementation of AJE showed a linear increase in the backfat thickness (BFT) at week 10, and there was no significant difference observed on the lean meat percentage. In summary, AJE supplementation had a beneficial effect on the growth performance, BFT, and also no effects on nutrient digestibility and fecal microbiota of the finishing pigs.

The embryo stage is critical for chicken development. Numerous studies have been conducted to clarify the dynamic changes and functions of various proteins and the composition of amino acids during embryo development. However, the physiological characteristics of extraembryonic fluid (allantoic and amniotic), especially allantoic fluid (AF), remain largely unexplored; furthermore, how information is transmitted from embryonic fluid is unknown. In this study, AF-derived exosomes ranging from 60 to 160 nm in diameter from fertilized eggs at 13 d of incubation of fast-growth chickens (WG chicken), medium-growth chickens (Silky N4 chicken), and slow-growth chickens (Huiyang Beard chicken) were isolated and purified by different ultra-centrifugations and further verified by transmission electron microscopy and a flow nano-analyzer. Expression of the exosomal positive biomarkers of ALIX and HSP70 as well as lack of the epithelium marker GRP78 was observed by Western blotting. In addition, small RNA sequencing revealed that AF-derived exosomes at 13 d of incubation contained a large number of known miRNAs (32.62%–65.83%). The top 10 most abundant and co-expressed miRNAs were primarily related to development, growth, and immunity. In addition, AF-derived exosomes promoted DF-1 cell migration. These findings broadened our understanding of the characteristic of AF-derived exosomes.

Growth performance, health status, and the radiated thermal temperatures of groups of piglets consuming non-medicated feed and in-feed antibiotics (chlortetracycline hydrochloride and tiamulin) were monitored continuously for up to 35 d, from weaning at 28 d of age until moving to commercial pens at approximately 63 d of age. There was a trend (P = 0.1) for body weight at the time of moving to be higher for the medicated animals. The average daily gain was higher in medicated piglets (P = 0.03). Radiated temperature variables were the maximum (T_max) and the average (T_mean) pig temperatures, and both were higher for the medicated piglets (P < 0.0001). A threshold temperature was derived to partition the image and derive the background radiated temperature that was used to obtain the expected radiated temperature of the pigs. Temperature data were adjusted for environmental effects by expressing the residual temperature. The residual for T_max (T_maxRes) and for T_mean (T_meanRes) were higher in medicated piglets (P < 0.0001). In-feed antibiotics were associated with increased growth rates and with an increase in radiated temperature variables, particularly those corrected for environmental conditions, possibly mediated through increased metabolic activity related to growth.

Six Holstein cows were used in a replicated 3 × 3 Latin Square design to investigate the effect of flaxseed meal on production performance and oxidative stability of milk enriched with n-3 fatty acids. Flaxseed oil was abomasally infused to all cows at 243 g·d⁻¹ which increased milk concentration of α-linolenic acid from 3.08 (pretrial) up to 53.0 mg·g⁻¹ fat. On a dry matter basis, dietary treatments were canola meal (CM; 165 g·kg⁻¹), flaxseed meal (FM; 165 g·kg⁻¹), and CM (165 g·kg⁻¹) + vitamin E (VE; 300 IU·kg⁻¹). Pre-planned contrasts were CM vs. FM and VE vs. FM. No difference was observed on dry matter intake, milk production, and yield of milk protein, and lactose for any of the contrasts evaluated. However, fat yield tended to be lower with FM relative to CM but was not different from VE. Milk enterolactone concentration was 6.8-fold greater with FM than with CM, whereas milk tocopherol was increased by 3.4-fold with VE relative to FM. Increased concentrations of enterolactone or tocopherol were not efficient to significantly modify the time course of appearance of propanal, hexanal, hept-cis-4-enal, and 1-octen-3-one in milk during storage evaluated for 10 d at 4 °C.

The study was aimed to investigate the changes in carcass traits, meat quality, muscle fiber characteristics, and liver function in pigs fed with high levels of fresh fish oil and oxidized fish oil. About 30 piglets were randomly assigned to receive basal diet plus 2% fish oil (LFO), basal diet plus 8% fish oil (HFO), or basal diet plus 8% oxidized fish oil (OFO) for 120 d. Pigs of the HFO and OFO group showed reduced carcass weight, dressing percentage, loin eye area, and increased yellowness of the longissimus dorsi muscle compared with LFO group (P < 0.05). Dietary HFO and OFO suppressed the relative expression levels of myosin heavy chain (MyHC) isoform (I and II a), glutathione peroxidase 4, and NAD(P)H: quinone oxidoreductase-1 and mitochondrial biogenesis in longissimus dorsi muscle (P < 0.05). Dietary HFO or OFO increased the serum aspartates aminotransferase, alanine aminotransferase, total bilirubin, direct bilirubin, oxidized low-density lipoprotein, liver index, and concentration of malondialdehyde (MDA) in liver (P < 0.05). In conclusion, high levels of fresh fish oil and oxidized fish oil have adverse effects on carcass traits, muscle fiber characteristics, and liver function, which may be partly due to the mitochondrial dysfunction and impaired antioxidative capacity.

The physiochemical properties and digestible, metabolizable, and predicted net energy contents in high-protein dried distillers’ grain (HiPro) were determined to assess the nutritive value for growing pigs. Twelve Yorkshire × Landrace barrows (initial body weight 25 ± 0.5 kg) were used in a partially replicated Latin square design over three periods (n = 7 or 8) and assigned to one of five experimental diets. In each period, pigs were adapted to diets for 7 d, followed by 5 d of total urine collection and fecal grab sampling. The experimental diets included a corn- and soybean-meal-based diet (CON) or diets containing dried distillers’ grains with solubles (DDGS) or HiPro to partially replace corn and soybean meal, without or with (i.e., DDGS+ and Hipro+) a multi-carbohydrase enzyme blend (0.05% inclusion). The HiPro ingredient contained half as much starch (2.6% vs. 5.2%; DM-basis), 20% more protein (32.5% vs. 27.1%), and had 14% greater water binding capacity versus DDGS. The digestible, metabolizable, and predicted net energy contents of the HiPro co-product were greater than DDGS for growing pigs (P < 0.05), but fibre-degrading enzymes were ineffective at improving energy values. The greater (available) energy and protein contents of HiPro make it a promising feed ingredient for inclusion in swine diets.

Corn production in Western Canada has increased with development of short-season varieties partially offsetting the use of barley as a silage and grain source. The current study evaluated effects of silage and cereal-grain source on subcutaneous fat (SCF) composition of finishing cattle. Steers (465 ± 28.0 kg) were assigned to 24 pens (12 steers per pen) in a 2 × 3 factorial design for an 89 d finishing study. Diets contained corn silage or barley silage at 8% [dry matter (DM)] combined either dry-rolled barley grain (BG; 86% of DM), corn grain (CG; 85% of DM), or an equal blend of BG and CG (85% of DM). Bone-in ribeyes were collected from four steers per pen, and subcutaneous fatty acids were analyzed by gas chromatography. Feeding dry-rolled CG increased dietary fat and 18:2n-6 concentrations, resulting in small increases in 18:2n-6, total n-6 fatty acids, and n-6/n-3 ratios in SCF (P < 0.05). There were no changes in proportions of major trans-18:1 isomers (t10-18:1 or t11-18:1), the main natural isomer of conjugated linoleic acid (cis9,t11–18:2), or n-3 fatty acids. Substitution of dry-rolled CG for BG in finisher diets can, therefore, be done without substantially altering the fatty acid composition of beef fat, including polyunsaturated fatty acid biohydrogenation products.

Two experiments were conducted to assess the effects of media pH and mixtures (SCEF) of live yeast [Saccharomyces cerevisiae (SC)] and lactic acid bacteria [Enterococcus faecium (EF)] on gas production (GP), dry matter disappearance (DMD), and volatile fatty acid (VFA) concentrations in batch culture using either high-forage (HF) or high-grain (HG) diets. Diets were evaluated in separate experiments, each as a complete randomized design with 2 (media pH 5.8 and 6.5) × 5 (control, three SCEF, monensin) factorial arrangement of treatments. The SCEF had varying ratios of SC:EF: 0:0 (control), 1.18:1 (SCEF1), 1.25:1 (SCEF2), and 1.32:1 (SCEF3), added on a log10 basis. For the HF diet, supplementation of SCEF had greater GP (P = 0.03) at pH 6.5 and greater DMD (P = 0.03) and VFA concentration (P < 0.01) at pH 5.8 and 6.5 than control. For the HG diet, acetate:propionate (A:P) ratio at pH 6.5 was greater (P = 0.05) for SCEF than control. Increasing ratio of SC to EF in SCEF linearly (P < 0.01) decreased GP and DMD and linearly increased acetate percentage at pH 6.5. These results suggest that optimizing the SC:EF ratio in a mixture of SCEF can help improve rumen fermentation.

A total of 10 cannulated barrows with an average body weight of 55.99 ± 3.73 kg were assigned to test diets containing either full-fat flaxseed (FFS), expeller-pressed flaxseed meal (expeller-FM), or solvent-extracted flaxseed meal (solvent-FM). The standardized ileal digestibility (SID) of methionine and threonine was higher (P < 0.05) in FFS than in expeller-FM. Similarly, the SID of crude protein (CP) and threonine was higher (P < 0.05) in FFS than that in solvent-FM. However, expeller-FM and solvent-FM had greater (P < 0.05) contents of SID CP, arginine, leucine, phenylalanine, aspartic acid, glutamic acid, and serine than FFS.

Pork belly quality indicators are economically relevant in the pork industry. Near-infrared (NIR) spectroscopy of the pork shoulder outer subcutaneous fat layer, belly flop angle, and subjective softness scores of the pork belly were measured (N = 144) to determine the accuracy of pork shoulder NIR spectroscopy as a predictor of pork belly softness. The NIR spectra hot carcass estimates explained over 80.0% variability in pork belly softness (80.5%–90.8%), with low prediction error, suggesting that NIR spectroscopy measured in the pork shoulder is an efficient and accurate indicator of pork belly softness to classify pork bellies for specific market demands.

A study was conducted to evaluate whether encapsulated active dried yeast (EDY), compared with non-protected active dried yeast (ADY) or antibiotics (ANT), improved immune response and blood metabolites of finishing beef heifers. Blood urea nitrogen was lower (P < 0.05) with supplemented ADY and mixture of ADY and EDY (MDY) compared with control. Supplementation of MDY also resulted in lower (P < 0.05) red blood cell distribution width than control. Lipopolysaccharide-binding protein was less (P < 0.05) for EDY than control, ANT, and ADY as well as cytokine concentration of interleukin-6 was less (P < 0.05) for MDY versus control.

This study examined the relationship of iodine value (IV) with carcass weight, fat depth, muscle depth, and predicted lean yield from 37 488 pork carcasses. Five IV categories were formed, which were defined as low (<64.99), medium-low (65.00–69.99), medium (70.00–74.99), medium-high (75.00–79.99), and high (>80.00). Correlation analysis indicated IV was weakly correlated (r ≤ 0.26; P < 0.05) with all carcass traits; however, the categorical analysis revealed that greater IV was associated with heavier weight and leaner carcasses. Segregation systems of pork carcasses based on IV should consider the relationships of IV with other carcass parameters before implementation.

The objective of this study was to evaluate associations of dry off management factors, milking activity and production data, and somatic cell count prior to dry off and early in the subsequent lactation of cows milked by automated systems. Data were collected for 342 cows from five farms, for two milk tests prior to dry off, and for the two milk tests post calving. The results suggest that the post-calving milking performance of cows milked by automated systems may be more associated with individual cow traits than with their dry off management.