The first known tetraploid amniote that reproduces through parthenogenetic cloning by individual females is named and described. The species originated through hybridization between Aspidoscelis exsanguis (triploid parthenogen) × Aspidoscelis inornata (diploid bisexual or gonochoristic species) in the laboratory. We compared multivariate morphological variation between two lineages that arose from separate F₁ hybrid zygotes in one clutch and among several generations in those lineages. The tetraploid species is also compared with its ancestral taxa, with two hybrids of A. exsanguis × A. inornata that were found in nature at two localities that are 100 km apart in southern New Mexico, and with three laboratory hybrid males. This will facilitate identification of field-caught tetraploids in the future.