The availability of shelter in karst areas affects the richness, abundance, and assemblage composition of bat species and may play an important role in movement dynamics, activity patterns, and foraging behavior. Our work in the midwestern region in the state of Minas Gerais, southeastern Brazil, showed a high richness of the bat fauna, given the anthropic impact caused by mineral exploration and agricultural activities, thus leaving vegetation islands over karst areas in a vast anthropized matrix. Sampling at 12 sites resulted in 1,444 captures of bats, representing 30 species. Our beta diversity partitioning analyses indicated a pattern of turnover, i.e. species replacement, suggesting that these vegetation fragments may function as ecological springboards or stopping points.