Mexico has a high amphibian diversity (360 species), but several regions of the country remain relatively poorly studied. We document the anuran fauna for the Marabasco River Basin, in Jalisco and Colima, Mexico. Between June and November 2012, we collected 13 species of anurans from 3 sites in the Marabasco, including Lithobates pustulosus, L. forreri, and L. neovolcanicus, which are all species with conservation priority. The anuran community of the Marabasco Basin could be threatened by mining activities and other anthropogenic activities. This is the first published report on the anuran community composition for this basin.