Sound production and spawning behavior has not been described in Cyprinella lepida, the Edwards Plateau shiner, although described for other species in the genus. This study documented acoustic signals associated with agonistic and courtship interactions produced by male C. lepida during the breeding season. Females did not produce sounds. Signals were monophasic consisting of one type of call (pulses). All sounds were non-harmonic, low frequency, and often occurred in trains called bursts. Courtship behaviors were characterized by differences in duration and dominant frequency. The exact mechanism of sound production in this species is unknown.