On 27 October 2004, in Sierra de las Minas Biosphere Reserve, Guatemala, we observed a strong-billed woodcreeper (Xiphocolaptes promeropirhynchus) chase and attempt to capture a mouse. The mouse jumped out of a bromeliad the woodcreeper was probing seconds earlier. If the woodcreeper was attempting to capture the mouse to eat it then it might have been opportunistically using a locally abundant food source although consumption of mammalian prey by woodcreepers has not been documented.