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1 July 2004 Molecular Phylogeny of Phyllopharyngean Ciliates and their Group I Introns
OONA L.O. SNOEYENBOS-WEST, JEFFREY COLE, ANNA CAMPBELL, D. WAYNE COATS, LAURA A. KATZ
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Abstract

We analyzed small subunit ribosomal DNA (ssu-rDNA) sequences to evaluate both the monophyly of the ciliate class Phyllopharyngea de Puytorac et al. (1974), and relationships among subclasses. Classifications based on morphology and ultrastructure divide the Phyllopharyngea into four subclasses, the Phyllopharyngia, Chonotrichia, Rhynchodia, and Suctoria. Our analyses of ssu-rDNA genealogies derived from sequence data collected from diverse members representing three of the four subclasses of Phyllopharyngea (Suctoria: Ephelota spp., Prodiscophyra collini, Acineta sp.; Phyllopharyngia: Chlamydodon exocellatus, Chlamydodon triquetrus, Dysteria sp.; and Chonotrichia: Isochona sp.) provide strong support for the monophyly of the Phyllopharyngea, and show that the Chonotrichia emerge from within the Phyllopharyngia. Based on this initial sampling, suctorian budding types are monophyletic, and exogenous budding appears to be basal to evaginative and endogenous budding. Further, we report the discovery of a group I intron at position 891 in the Suctoria Acineta sp. and Tokophrya lemnarum, and a second group I intron at position 1506 in T. lemnarum. These introns represent only the second examples of group I introns in a ciliate ribosomal gene, since the discovery of ribozymes in the LSU rRNA gene of Tetrahymena thermophila. Phylogenetic analyses of Group I introns suggest a complex evolutionary history involving either multiple loses or gains of introns within endogenously budding Suctoria.

OONA L.O. SNOEYENBOS-WEST, JEFFREY COLE, ANNA CAMPBELL, D. WAYNE COATS, and LAURA A. KATZ "Molecular Phylogeny of Phyllopharyngean Ciliates and their Group I Introns," The Journal of Eukaryotic Microbiology 51(4), 441-450, (1 July 2004). https://doi.org/10.1111/j.1550-7408.2004.tb00392.x
Received: 16 January 2004; Accepted: 16 March 2004; Published: 1 July 2004
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KEYWORDS
Chonotrichia
Ciliophora
group I introns
molecular genealogy
Phyllopharyngea
ssu-rDNA
Suctoria
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