BioOne.org will be down briefly for maintenance on 17 December 2024 between 18:00-22:00 Pacific Time US. We apologize for any inconvenience.
How to translate text using browser tools
1 January 2002 Serological Affinities of the Oyster Pathogen Perkinsus marinus (Apicomplexa) with Some Dinoflagellates (Dinophyceae)
DAVID BUSHEK, CHRISTOPHER F. DUNGAN, ALAN J. LEWITUS
Author Affiliations +
Abstract

The protozoan oyster pathogen Perkinsus marinus is classified in the phylum Apicomplexa, although molecular-genetic and ultrastructural evidence increasingly concur on its closer phylogenetic relationship with the dinoflagellates. To test for evidence of serological epitopes common to P. marinus and dinoflagellates, we probed 19 free-living and 8 parasitic dinoflagellate, or dinoflagellate-like, species for cross-reactivity with polyclonal antibodies to P. marinus. Three of 19 free-living dinoflagellates (16%), and 7 of 8 parasitic dinoflagellates (88%) were labeled by anti-P. marinus antibodies. In reciprocal immunoassays using polyclonal antibodies to the Hematodinium sp. dinoflagellate parasite of Norway lobsters, Nephrops norvegicus, P. marinus and the same 7 parasitic dinoflagellates labeled by anti-P. marinus antibodies, were again labeled. The dinoflagellate-like parasite of prawns Pandalus platyceros was not labeled by either antibody reagent. These reciprocal results confirm the presence of shared antibody-binding epitopes on cells of P. marinus and several dinoflagellates. The apparent widespread serological affinity between P. marinus and the parasitic dinoflagellates suggests a closer phylogenetic link to the syndinean dinoflagellate lineage. The consistent failure of the dinoflagellate-like prawn parasite to bind either antibody reagent shows that this parasite is serologically distinct from both P. marinus and Hematodinium-species parasitic dinoflagellates.

DAVID BUSHEK, CHRISTOPHER F. DUNGAN, and ALAN J. LEWITUS "Serological Affinities of the Oyster Pathogen Perkinsus marinus (Apicomplexa) with Some Dinoflagellates (Dinophyceae)," The Journal of Eukaryotic Microbiology 49(1), 11-16, (1 January 2002). https://doi.org/10.1111/j.1550-7408.2002.tb00333.x
Received: 26 October 2000; Accepted: 25 September 2001; Published: 1 January 2002
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

KEYWORDS
Antibody cross-reaction
dermo
epitope sharing
Hematodinium, immunoassay
oyster disease
parasitic dinoflagellates
protozoan phylogeny
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top