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1 November 2000 The Role of Cortical Geometry in the Nuclear Development of Tetrahymena thermophila
JACEK GAERTIG, ERIC S. COLE
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Abstract

Vegetative cells were subjected to electrofusion and the resulting heteropolar doublets were then mated to normal single cells and followed throughout conjugation using cytological and genetic techniques. The unique cyto-geometry created in a heteropolar doublet—a continuous cytoplasmic compartment bounded by two anterior poles and sharing a fused posterior pole at midbody, and the potential for two conjugal exchange junctions—resulted in instructive perturbations of nuclear behavior. Our results indicate that the course of nuclear development is strongly dependent on the cortical geometry of conjugating cells. Specifically, 1) continuation of development after meiosis requires an established conjugal junction; 2) after pronuclear exchange, pronuclei are subjected to attractive forces; and 3) products of the second postzygotic division are actively positioned near the posterior region of the cell cortex where they develop into micronuclei.

JACEK GAERTIG and ERIC S. COLE "The Role of Cortical Geometry in the Nuclear Development of Tetrahymena thermophila," The Journal of Eukaryotic Microbiology 47(6), 590-596, (1 November 2000). https://doi.org/10.1111/j.1550-7408.2000.tb00095.x
Received: 27 September 1999; Accepted: 10 August 2000; Published: 1 November 2000
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KEYWORDS
cell cycle
Ciliates
Conjugation
electrofusion
meiosis
mitosis
parabiosis
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