Habitat associations of two species of congeneric web-building wolf spiders were examined in their zone of syntopy on the Lake Wales Ridge in Florida. The species differed in use of all of the habitat variables measured. The geographically widespread species, Sosippus floridanus Simon, 1898, associated with habitat features typical of scrubby flatwoods and human disturbed areas, which are found throughout the Florida peninsula. The endemic species, S. placidus Brady, 1972, associated with a scrub oak habitat that is restricted to the Lake Wales Ridge. Biogeographic implications of these differences are discussed.