Urbanization is associated with a variety of anthropogenic impacts that alter aquatic ecosystems and could affect riparian web-spinning spiders. The objective of this study was to evaluate how changes in web structural features and body condition of a horizontal orb-weaver are associated with surrounding levels of urbanization. Along an urban watershed in Puerto Rico, we found a significant negative relationship between the capture area of webs and in the body condition of spiders with increasing levels of surrounding impervious surface. We propose that these changes in web structure and body condition are associated with variations in the diversity and quality of prey, as well as the loss of riparian substrate in more heavily urban areas.