Two new scorpion species are described from Colombia: Ananteris arcadioi sp. nov. from Meta Department and Ananteris dorae sp. nov. from Nariño Department. These new species raise to 62 the number of known species of Ananteris, eight of which are found in Colombia. Some comments on the taxonomy of Colombian Ananteris are included and some characters are proposed to define species whereas the usefulness of others is briefly discussed. A map with the known distribution of the different species in Colombia, a table for some characters, and a key for the identification of the Colombian species of the genus are included.