Surface ultrastructure of labial and maxillary cuspules was studied in eight species of seven different genera of Theraphosidae. Cuspule ornamentation was observed through SEM images and comparisons were made among labial and maxillary cuspules of different species and different zones of each cuspule. Ornamentation patterns were different on the anterior face of the cuspule with respect to the posterior face. A significant correlation analysis between cuspule size and body size was found. The systematic use and the probable functions of the cuspules are discussed.