False map turtles (FMT, Graptemys pseudogeographica) are found along the Missouri and Mississippi Rivers throughout the United States but are a protected species in South Dakota (SD). Graptemys pseudogeographica blood samples were collected from two sites above and below the Gavins Point Dam along the Missouri River near Yankton, SD for the purposes of creating a microsatellite library. We isolated and characterized ten microsatellite loci in G. pseudogeographica, and only one locus (FMT201) shows as deficit of heterozygotes relative to expectations.