To reduce the cost of educational materials needed by students, we generated a no-cost open educational resources (OER) textbook for an undergraduate level cell biology course. Cell biology is part of the curriculum in various undergraduate degrees for science, technology, engineering, and math majors, therefore generation of OER targets a large population of students. In addition to cost reduction, we unified the textbook content with course learning outcomes to ensure that the students have a clear understanding of course goals. To assess student perceptions of OER materials and their experiences related to learning outcomes, surveys were administered to students enrolled in sections of cell biology using a traditional textbook and those enrolled in sections using the OER. Data showed positive student attitudes about learning, accessibility, and cost of OER, supporting the use of no-cost OER in undergraduate classrooms and beyond.