In this paper, a new species of Kuzinellus Wainstein, K. gabonensis sp. nov., is described based on specimens collected in Gabon. This new species is morphologically similar to K. querellus (Ueckermann & Loots), but it can be distinguished mainly by the knobbed tip of the dorsal setae Z4, spermatheca shape and by its possession of more than 15% divergence in the DNA barcode region of COI. Additionally, Kuzinellus querellus is reported for the first time from Kenya and accompanied by a redescription of morphological and DNA barcode data.