Here we report the detection of Candidatus Rickettsia tarasevichiae from a tick collected from a human patient through molecular method. We amplified and sequenced rickettsial citrate synthase encoding gene (gltA), 190_kDa outer protein gene (ompA), and 120_kDa outer protein gene (ompB). Sequencing results showed that its gltA gene and ompA gene shared 100% and 96% sequence similarities with Candidatus R. tarasevichiae gltAgene (GenBank: KT899085) and R. canadensis ompB gene (GenBank: KT899079), respectively. On the other hand, DNA extracted from the patient's serum shared 98% sequence similarities with R. japonica (GenBank: AF123713). Thus, we identified Candidatus R. tarasevichiae from tick and R. japonica from patient serum in this study.