Acaroid mites are among the economically important pests infesting various plant materials. In an attempt to investigate the status of the mite pest prevalence in Chinese traditional medicinal herbal materials, we collected 1,810 samples of crude herbs from certain traditional Chinese drugstores in Huainan City, Anhui Province, China. In total, 45 species of mites belonging to 25 genera and 10 families were identified. The findings revealed that 64% (1,165/1,810) of the samples were infested by mites, suggesting that acaroid mites are common in the medicinal herbs in stores in Anhui Province. Since some mites are well documented as allergen producers, greater concerns and important measures should be paid and taken to prevent the mites from infecting the herbs associated with public health.