Two new oribatid mite species, Machadobelba longiciliata sp. nov. (Machadobelbidae) and Campachipteria brevisetosa sp. nov. (Achipteriidae), are described from upper organic soil layer in mostly undisturbed rain forest of Podocarpus National Park, Ecuador. Machadobelba longiciliata sp. nov. is most similar to Machadobelba descombesi Mahunka, however it differs from the latter by the structures of sensilli and median notogastral tubercles, length of notogastral setae and presence of porose areas in anogenital region. Campachipteria brevisetosa sp. nov. is most similar to Campachipteria sibirica (Krivolutsky et Grishina), however it differs from the latter by the structures of sensilli and lamellar setae, and length of process of pteromorphs. The genus Campachipteria is recorded for the first time in Ecuador.