Neopodocinum caputmedusae (Berlese, 1908), Macrocheles peniculatus Berlese, 1918 and M. recki Bregetova & Koroleva, 1960 are recorded for the first time from Iran. The genus Neopodocinum Oudemans, 1902 is also new to the Iranian fauna. Neopodocinum caputmedusae was collected from a beetle (Copris sp., Scarabaeidae; Coleoptera) and M. peniculatus and M. recki were found in the soil. A key is provided to the adult females of the known species of Macrochelidae of Iran.