Tick-infested bats, rodents and marsupials were captured along the Iquitos-Nauta highway, Loreto Department, in the Peruvian Amazon. The only ticks found on Chiroptera were members of the Ornithodoros hasei (Schulze, 1935) species group, collected from Noctilio albiventris Desmarest, 1818, Rhinophylla pumilio Peters, 1865 and Myotis albescens (É. Geoffroy, 1806). Rhinophylla pumilio is a new host and M. albescens was confirmed as a host for the O. hasei group. Among Rodentia, Oecomys bicolor (Tomes, 1860), Oligoryzomys microtis (Allen, 1916), Hylaeamys perenensis (Allen, 1901) and Hylaeamys yunganus (Thomas, 1902) were infested with larvae (O. bicolor) or nymphs (other three rodents) of Ixodes luciae Sénevet, 1940; all are new hosts for I. luciae. Tick infestation was more diverse among the Didelphimorphia, where a nymph and larva of Amblyomma sp. were collected on Philander opossum (Linnaeus, 1758), a nymph of Amblyomma sp. on Caluromys lanatus (Olfers, 1818), and a larva of Ixodes pararicinus Keirans & Clifford, 1985 on Monodelphis adusta (Thomas, 1897). Monodelphis adusta is a new host for I. pararicinus. Caluromys lanatus, Marmosops sp., Metachirus nudicaudatus (Desmarest, 1817), Micoureus sp., Philander andersoni (Osgood, 1913) and P. opossum were parasitized with larvae (Micoureus sp.) or adults (all other didelphids) of I. luciae. With the exception of P. opossum, all these marsupials are new hosts for I. luciae.