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1 July 2024 Pathogenic and Molecular Characterization of Two Mexican Isolates of Entomopathogenic Nematodes against Fall Armyworm Larvae and Their Effect on Chrysoperla carnea
Oscar Giovanni Gutiérrez-Cárdenas, María Guadalupe Valdovinos-Torres, Alan Roberto Robledo-Vega, Luis Enrique Flores-Pantoja, Ma. Guadalupe Sánchez-Saavedra, Gerardo Arias-Robledo, Isaac Zepeda-Jazo
Author Affiliations +
Abstract

This work focused on studying these interactions through laboratory bioassays to characterize the effects of native isolates of entomopathogenic nematodes (EPNs) on the larvae of Spodoptera frugiperda (Smith) at the pathogenic and molecular levels. In addition, through the ingestion of EPNs-treated diet, their non-target effects on larvae of the generalist predator Chrysoperla carnea (Stephens) were also studied. Molecular characterization revealed that the EPN isolates tested corresponded to Oscheius myriophilus (Poinar) and Rhabditis spp. Infection of the S. frugiperda larvae with both EPNs produced high mortality (up to 56.52%) with different results in the rate of death according to median time to death analysis. Similarly, ingestion by the predator mediated by a treated diet triggered lethal effects on the preimaginal parameters of C. carnea. In this study, it was demonstrated that the use of native isolates of EPNs for the control of S. frugiperda is a strategy that can be applied for the management of this insect.

Este trabajo se centró en estudiar estas interacciones mediante bioensayos de laboratorio para caracterizar los efectos de aislamientos nativos de nematodos entomopatógenos (NEPs) sobre larvas de Spodoptera frugiperda (Smith) a nivel patogénico y molecular. Además, a través de la ingestión de dieta tratada con NEPs se estudió su efecto no objetivo sobre larvas del depredador generalista Chrysoperla carnea (Stephens). La caracterización molecular reveló que los aislados de NEPs analizados corresponden a Oscheius myriophilus (Poinar) y Rhabditis spp. La infección de larvas de S. frugiperda con ambos NEPs produjo una alta mortalidad (hasta 56.52%) con resultados diferentes en la velocidad de muerte de acuerdo con el análisis de la mediana del tiempo de mortalidad. De manera similar, la ingestión por parte del depredador mediada por dieta tratada desencadenó efectos letales en parámetros preimaginales de C. carnea. En este estudio, se demostró que el uso de aislamientos nativos de NEPs para el control de S. frugiperda es una estrategia que puede ser aplicada para el manejo del insecto.

Oscar Giovanni Gutiérrez-Cárdenas, María Guadalupe Valdovinos-Torres, Alan Roberto Robledo-Vega, Luis Enrique Flores-Pantoja, Ma. Guadalupe Sánchez-Saavedra, Gerardo Arias-Robledo, and Isaac Zepeda-Jazo "Pathogenic and Molecular Characterization of Two Mexican Isolates of Entomopathogenic Nematodes against Fall Armyworm Larvae and Their Effect on Chrysoperla carnea," Southwestern Entomologist 49(2), 589-601, (1 July 2024). https://doi.org/10.3958/059.049.0218
Published: 1 July 2024
KEYWORDS
Oscheius myriophilus
Oscheius myriophilus
Rhabditis spp.
Rhabditis spp.
Spodoptera frugiperda
Spodoptera frugiperda
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