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9 May 2024 Selection of Native Beauveria bassiana Strains for Formulation by Coacervation and the Effect of Temperature and UV Radiation on Their in vitro Stability
Jairo H. Alfaro-Álvarez, Fatima Lizeth Gandarilla-Pacheco, Isela Quintero-Zapata, Magda Patricia Vargas-Pérez, Claudio Guajardo- Barbosa, Hugo A. Luna-Olvera, Myriam Elías-Santos
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Abstract

The objective of this study was to determine the effects of temperature and ultraviolet radiation (UV-B) on a microencapsulated formulation created by coacervation using the conidia of native Beauveria bassiana strains as the active ingredient. A strain of B. bassiana (HIB-15) was selected in a trial with Bemisia tabaci nymphs where we obtained 77% mortality. The viability of the microencapsulated conidia was 96% at 45 °C, while the effect of UV-B radiation was negative since the viability decreased to 20% at the time of exposure. This shows that the formulation provided protection with respect to temperature but did not protect against the effect of radiation.

El objetivo de este estudio fue determinar los efectos de la temperatura y la radiación ultravioleta (UV-B) en una formulación microencapsulada creada por coacervación utilizando los conidios de cepas nativas de Beauveria bassiana como ingrediente activo. Se seleccionó una cepa de B. bassiana (HIB-15) en un ensayo con ninfas de Bemisia tabaci donde se obtuvo un 77% de mortalidad. La viabilidad de los conidios microencapsulados fue del 96% a 45 °C, mientras que el efecto de la radiación UV-B fue negativo ya que la viabilidad disminuyó al 20% al momento de la exposición. Esto muestra que la formulación proporcionó protección con respecto a la temperatura, pero no protegió contra el efecto de la radiación.

Jairo H. Alfaro-Álvarez, Fatima Lizeth Gandarilla-Pacheco, Isela Quintero-Zapata, Magda Patricia Vargas-Pérez, Claudio Guajardo- Barbosa, Hugo A. Luna-Olvera, and Myriam Elías-Santos "Selection of Native Beauveria bassiana Strains for Formulation by Coacervation and the Effect of Temperature and UV Radiation on Their in vitro Stability," Southwestern Entomologist 49(2), 658-672, (9 May 2024). https://doi.org/10.3958/059.049.0210
Published: 9 May 2024
KEYWORDS
Beauveria bassiana
Beauveria bassiana
coacervación
coacervation
conidia
conidios
radiación
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