Two new species of Boletina Staeger fungus gnat were discovered in western Michigan. Adult males of both species flew to a Malaise trap in spring and a second generation of one species flew during the transition from late summer to autumn. The habitat was an ecotone between swamp and second-growth forest dominated by black ash (Fraxinus nigra Marshall), paper birch (Betula papyrifera Marshall), red maple (Acer rubrum L.), and black cherry (Prunus serotina Ehrhart). Females and juvenile stages of Boletina dentata Taber and Boletina tricinctoides Taber are either unknown or have not been described.