We report new rearing records for five species of Drosophilidae (Diptera) in North America. Chymomyza amoena (Loew), previously reared from tree nuts, was reared from a gall of Amphibolips cookii Gillette (Hymenoptera: Cynipidae) collected from Quercus rubra L. (Fagaceae). Borodinia dentata (Raf.) P.J. Alexander and Windham and Cardamine diphylla (Michx.) Alph. Wood (Brassicaceae) are new hosts for the leafminers Scaptomyza flava (Fallén) and S. montana Wheeler, respectively. The saprophagous species S. pallida (Zetterstedt) was reared from leaf mines, galls, and leaf rolls caused by other fly and moth larvae. Stegana vittata (Coquillett) is newly reported as a stem borer of Amphicarpaea bracteata (L.) Fernald (Fabaceae).