Stream fish survey data were analyzed to describe patterns of fish distributions in wadeable streams (primarily 1^st–4^th order) in Connecticut. Species occurrence within the United States Geological Survey 8-digit hydrologic unit code watersheds were used to aggregate similar watersheds into stream fish faunal regions. Within each identified region, multivariate analyses were used to identify major fish assemblage types and associate stream habitat with assemblage types. The analyses revealed an eastern and western faunal region defined primarily by distribution of a few native species. Native species associated with the western watersheds were: Semotilus atromaculatus (Creek Chub), Exoglossum maxillingua (Cutlips Minnow), and Cottus cognatus (Slimy Sculpin). Native fishes associated with the eastern watersheds were: Erimyzon oblongus (Creek Chubsucker), Esox niger (Chain Pickerel), and Esox americanus (Redfin Pickerel). Inclusion of non-indigenous species in the analyses resulted in a similar east–west grouping of watersheds. Five and four assemblage types were identified in the eastern and western faunal regions, respectively. Both regions harbored 3 fluvial assemblages defined longitudinally from headwater streams to larger wadeable streams and a macro-habitat generalist assemblage inhabiting streams with proportionately more pool habitat, but taxonomic membership and indicator species rankings among assemblages were not necessarily identical between the regions. A distinct assemblage dominated by Redfin Pickerel was recognized only in the eastern region. Streams in the western region were generally higher in elevation and colder in water temperature. The discovery and description of eastern and western fish faunal regions and their fish assemblage types will be useful in stratifying the biological monitoring of streams and other aquatic resource management actions in Connecticut.