Utility of a Fecal Real-time PCR Protocol for Detection of Mycobacterium bovis Infection in African Buffalo (Syncerus caffer)

Annette Roug; Claire Geoghegan; Elizabeth Wellington; Woutrina A. Miller; Emma Travis; David Porter; David Cooper; Deana L. Clifford; Jonna A. K. Mazet; Sven Parsons

doi:10.7589/2013-07-159

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Annette Roug,^1,* Claire Geoghegan,² Elizabeth Wellington,³ Woutrina A. Miller,¹ Emma Travis,³ David Porter,³ David Cooper,⁴ Deana L. Clifford,^1,5 Jonna A. K. Mazet,¹ Sven Parsons⁶

¹1 Wildlife Health Center & One Health Institute, School of Veterinary Medicine, 1089 Veterinary Medi
²2 Mammal Research Institute, Department of Zoology and Entomology, University of Pretoria, Private b
³3 School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK
⁴4 Ezemvelo KZN Wildlife Game Capture Unit, PO Box 13053 Cascades, Pietermaritzburg 3202, South Afric
⁵5 California Department of Fish and Wildlife, 1701 Nimbus Road, Suite D, Rancho Cordova, California
⁶6 Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 19063, Tygerberg, 7505, S

^*Corresponding author. E-mail: aroug@ucdavis.edu

Abstract

A real-time PCR protocol for detecting Mycobacterium bovis in feces was evaluated in bovine tuberculosis–infected African buffalo (Syncerus caffer). Fecal samples spiked with 1.42×10³ cells of M. bovis culture/g and Bacille Calmette–Guérin standards with 1.58×10¹ genome copies/well were positive by real-time PCR but all field samples were negative.

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Annette Roug, Claire Geoghegan, Elizabeth Wellington, Woutrina A. Miller, Emma Travis, David Porter, David Cooper, Deana L. Clifford, Jonna A. K. Mazet, and Sven Parsons "Utility of a Fecal Real-time PCR Protocol for Detection of Mycobacterium bovis Infection in African Buffalo (Syncerus caffer)," Journal of Wildlife Diseases 50(1), 140-142, (1 January 2014). https://doi.org/10.7589/2013-07-159

Received: 10 July 2013; Accepted: 1 July 2013; Published: 1 January 2014

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