A species composition analysis of regions of Kazakhstan was carried out by examining the genetic diversity of the blood-feeding mosquito species Anopheles maculipennis complex in the northern and western regions of Kazakhstan. Mosquitoes of the species Anopheles maculipennis complex are of significant interest due to their role as vectors of malaria and other vector-borne diseases. A comprehensive genetic analysis was conducted using molecular markers. It revealed notable genetic variations within mosquito populations in some Kazakhstan regions, shedding light on the potential implications for malaria control efforts and providing valuable insights for future research in the field of vector biology and disease epidemiology.