Gill and heart tissues from the abalone Haliotis asinina were cultured in vitro using Leibovitz L15 medium supplemented with insulinlike growth factor and basic fibroblast growth factor. Culture conditions at a pH of 7.8–8.0, a temperature of 27–28°C, and 31–32% salinity yielded cell growth expansion from the explanted tissue. Dissociated, floating spherical cells were found along with spindle-shaped fibroblastlike cells, forming a cell monolayer. This study provides basic knowledge in the development of in vitro culture technology of abalone tissues and is the first report on H. asinina cell culture.