How to translate text using browser tools
23 January 2024 Efficient RNAi knockdown at 20 °C in Aedes albopictus (Diptera: Culicidae)
Mara Heilig, Peter A. Armbruster
Author Affiliations +
Abstract

RNA interference (RNAi) is a conserved cellular pathway found in nearly all eukaryotes that leads to the silencing of target mRNAs. Using RNAi as a mechanism to knockdown specific genes has enabled functional and reverse genetics studies in a wide range of eukaryotes. Previous work suggests that RNAi is inhibited at lower temperatures, potentially limiting the possibility to perform knockdown studies on ecologically relevant phenotypes that are only expressed at low temperatures. To determine whether RNAi is inhibited at low temperatures in Aedes albopictus (Skuse), we injected mosquitoes reared at 20 ± 1 °C, with dsRNA targeting yellow-g2 and compared knockdown efficacy to mosquitoes injected at 26.5 ± 1 °C. Our results demonstrate efficient knockdown at both temperatures, thereby establishing the feasibility of RNAi for functional genetic studies in A. albopictus at low temperatures.

Mara Heilig and Peter A. Armbruster "Efficient RNAi knockdown at 20 °C in Aedes albopictus (Diptera: Culicidae)," Journal of Medical Entomology 61(2), 508-511, (23 January 2024). https://doi.org/10.1093/jme/tjae002
Received: 16 October 2023; Accepted: 13 January 2024; Published: 23 January 2024
JOURNAL ARTICLE
4 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

KEYWORDS
Aedes albopictus
diapause
low temperature
RNAi
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top