Aedeomyia squamipennis (Lynch Arribalzaga) is a tropical mosquito (Diptera: Culicidae) found throughout most of the American Tropics, from eastern Mexico through Argentina, including several Caribbean islands. Larvae are typically associated with bodies of water with dense growths of aquatic vegetation, particularly Pistia stratiotes L., water lettuce. Adult females feed predominantly on the blood of birds and seek hosts in forest canopies. Aedeomyia squamipennis is considered an important vector of Gamboa virus and avian malaria, and is also suspected of transmitting Venezuelan equine encephalitis virus. Collections from Florida City, FL, near the southern tip of the Florida peninsula, yielded larvae, adult males, and females of Ad. squamipennis, constituting a new genus and species record for Florida and the United States. The widespread availability of larval habitat and suitable hosts in Florida will likely lead to expansion of Ad. squamipennis in Florida, and perhaps into neighboring states. In South America, Ad. squamipennis is found as far south as Buenos Aires, Argentina, at ~35° S latitude, which is equivalent in N latitude to coastal North Carolina. The northern limit of expansion of Ad. squamipennis in North America will likely be limited by winter temperatures and availability of larval habitat.