The effect of cryopreservation and subsequent xenotransplantation on the follicular reserve of the canine ovary by using non obese diabetic-severe combined immunodeficient (NOD-SCID) mice was examined. Vitrified-warmed canine ovarian tissues were placed into the ovarian bursa of mice, and then were removed and subjected to histological examination at 4 weeks after the transplantation. Over 30% of primordial follicles and 65% of early primary follicles survived after cryopreservation. However, regardless of breed or age, percentages of survived primordial follicles and early primary follicles after the transplantation ranged from 0–7% and from 0–15%, respectively. These results indicate that the majority of primordial follicles and early primary follicles in vitrified-warmed canine ovarian tissues disappear after xenotransplantation. Further studies will be required to be able to enhance the survival of transplanted cryopreserved ovarian follicles in canine.
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1 April 2009
Follicular Loss of the Cryopreserved Canine Ovary after Xenotransplantation
Takako Ishijima,
Yasuyuki Abe,
Hiroshi Suzuki
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Journal of Mammalian Ova Research
Vol. 26 • No. 1
April 2009
Vol. 26 • No. 1
April 2009
canine
cryopreservation
ovary
Transplantation