Fecundity is defined as the number of progeny produced by an organism. In our study, we used three different experimental settings to measure the fecundity of rugose spiraling whitefly (Aleurodicus rugioperculatus Martin), a recent invasive insect in Florida, on Strelitzia nicolai host plants. The adult longevity was significantly different among treatments, with females surviving significantly longer in groups in whole-plant cages and individual females in clip cages compared with individual females in whole-plant cages. Females laid 34.5 ± 8.4, 156.4 ± 18.3, and 225.2 ± 18 eggs, on average, when kept individually in whole-plant cages, in a group in whole-plant cages, and individually in clip cages, respectively. In all treatments, there was a significant correlation between the longevity and fecundity of females. Males emerged earlier than females in all treatments. Virgin females produced only males in the F1, while mated females produced both males and females, which suggests an arrhenotokous parthenogenesis. The egg-to-adult survival of the whitefly was 29 ± 4%, and the highest mortality occurred at the crawler stage when reared on S. nicolai. The results showed a significant effect of aggregation and cage setting on longevity and fecundity of this whitefly. To conclude, the experimental setting of the group fecundity treatment is more similar to what occurs in the landscape. Such a significant effect of cage setting and aggregation on the life-history parameters should be always taken into account when designing experiments or interpreting results.