How to translate text using browser tools
14 June 2008 An efficient method for in vitro regeneration of leafy spurge (Euphorbia esula L.)
Bin Xu, Wenhao Dai, Wun S. Chao
Author Affiliations +
Abstract

Leafy spurge (Euphorbia esula L.) is a perennial, invasive weed used as a model to study invasive plant behavior, because molecular tools (such as a deep expressed sequence tag database and deoxyribonucleic acid microarrays) have been developed for this species. However, the lack of effective in vitro regeneration and genetic transformation systems has hampered molecular approaches to study leafy spurge. In this study, we describe an efficient in vitro regeneration system. Three highly regenerative lines were selected by screening the in vitro regeneration capabilities of stem explants of 162 seedlings. The effects of various culture conditions on in vitro regeneration were then evaluated based on explant competence to form calluses and shoots. High rates of shoot regeneration can be obtained using a growth medium containing 1× woody plant basal medium and 1× Murashige and Skoog (MS) basal salts, 1× MS vitamins, 1.11 μM 6-benzylaminopur-ine, 1.97 μM indole-3-butyric acid, and 3% sucrose, pH 5.6–5.8. After 30 d culture, multiple shoots formed either directly from the stem or indirectly from the callus. This method is a requisite for the development of genetic transformation systems for leafy spurge and may be used to develop in vitro regeneration techniques for other species in the Euphorbiaceae.

Bin Xu, Wenhao Dai, and Wun S. Chao "An efficient method for in vitro regeneration of leafy spurge (Euphorbia esula L.)," In Vitro Cellular and Developmental Biology - Plant 44(6), 548-556, (14 June 2008). https://doi.org/10.1007/s11627-008-9139-9
Received: 8 March 2007; Accepted: 23 April 2008; Published: 14 June 2008
JOURNAL ARTICLE
9 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

KEYWORDS
Euphorbiaceae
High salt requirement
invasive weed
Selection for regeneration
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top