A procedure for the micropropagation of Chimonanthus praecox (L) Link, wintersweet, has been developed using buds from adult trees excised in spring. Shoot cultures established on Murashige and Skoog (1962) medium supplemented with 0.5 mg l−1 6-benzyladenine (BAP) and 0.1 mg l−1 indole-3-butyric acid (IBA) were difficult to maintain in vitro through extended periods of time due to browning of the medium, shoot and leaf necrosis, and hyperhydricity. A treatment combining the use of 0.1% w/v activated charcoal and addition of a double phase agar-solidified/liquid medium improved propagation, enabling a successful in vitro propagation scheme to be developed. Optimal shoot multiplication occurred on medium containing 0.5 mg l−1 BAP, and rooting on medium with 2.0 mg l−1 IBA for 7 d, followed by transfer to hormone-free medium. Rooted plantlets were easily acclimated in a glasshouse and replanted and cultured outdoors.
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28 May 2008
In vitro propagation of Chimonanthus praecox (L.), a winter flowering ornamental shrub
B. Kozomara,
B. Vinterhalter,
Lj Radojević,
D. Vinterhalter
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In Vitro Cellular and Developmental Biology - Plant
Vol. 44 • No. 2
March 2008
Vol. 44 • No. 2
March 2008
Activated charcoal
Chimonanthus praecox
Double phase culture medium
micropropagation
Wintersweet