Agrobacterium rhizogenes strain K599 (pRi2659), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species, including numerous soybean (Glycine max) cultivars. Because Agrobacterium-mediated transformation of soybean remains challenging and labor intensive, K599 appeared a suitable progenitor for new agrobacteria strains for plant transformation. In this paper, we report the disarming and sequencing of pRi2659 and the usefulness of the resulting disarmed strain in plant transformation studies of Arabidopsis thaliana, maize (Zea mays), tomato (Lycopersicon esculentum), and soybean (G. max).
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19 September 2007
Disarming and sequencing of Agrobacterium rhizogenes strain K599 (NCPPB2659) plasmid pRi2659
S. Luke Mankin,
D. Steven Hill,
Paula M. Olhoft,
Effie Toren,
Allan R. Wenck,
Lawrence Nea,
Liqun Xing,
Jeffrey A. Brown,
Huihua Fu,
Lesley Ireland,
Hongmei Jia,
Helke Hillebrand,
Todd Jones,
Hee-Sook Song,
P. Ozias-Akins
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In Vitro Cellular and Developmental Biology - Plant
Vol. 43 • No. 6
November 2007
Vol. 43 • No. 6
November 2007
Disarmed
Hairy root
K599
plant transformation