Hydrastis canadensis L. (Goldenseal) is an endangered medicinal plant used in the treatment of many ailments, such as gastrointestinal disturbances, urinary disorders, hemorrhage, skin, mouth and eye infections, and inflammation. Commercial preparations of wild-harvested goldenseal were found to contain heavy metal contaminants including aluminum (848 μg g−1), cadmium (0.4 μg g−1), lead (18.7 μg g−1), and mercury (0.1 μg g−1). As well, goldenseal is an endangered species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II. Therefore, the practice of wild-harvest is actually decimating natural populations of goldenseal and endangering its genetic diversity. In vitro propagation protocol by tissue culture was developed for producing high-quality tissues of goldenseal. Significantly more de novo regeneration was induced on stem explants of 3-mo.-old plants cultured on a medium containing 10 μM 6-benzylaminopurine (BA) (22 regenerants per explant) than any other treatment. Subculture of the regenerants on a medium devoid of growth regulators resulted in the development of complete plants that were acclimatized and thrived in standard greenhouse conditions. The plants regenerated in vitro contained the lowest levels of heavy metals. The findings of this study provide the first evidence that heavy metal contaminants bioaccumulate in goldenseal tissues and also provide a method for germplasm conservation, mass multiplication, and production of goldenseal tissues free from abiotic contamination.
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1 January 2004
GOLDENSEAL (HYDRASTIS CANADENSIS L.): IN VITRO REGENERATION FOR GERMPLASM CONSERVATION AND ELIMINATION OF HEAVY METAL CONTAMINATION
CHUN-ZHAO LIU,
SUSAN J. MURCH,
JINESH C. JAIN,
PRAVEEN K. SAXENA
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In Vitro Cellular and Developmental Biology - Plant
Vol. 40 • No. 1
January 2004
Vol. 40 • No. 1
January 2004
Heavy metal contamination
Hydrastis canadensis L
Medicinal plant
Plant regeneration