Glutathione S-transferases (GSTs) in insects are widely known for their role in the detoxification of both endogenous and xenobiotic compounds. Grape phylloxera, Daktulosphaira vitifoliae (Fitch) (Hemiptera: Phylloxeridae) is a serious grape pest, which causes great economic damage in vineyards, and has currently spread throughout the world. In this study, eight putative GST genes were identified by analyzing the transcriptomes of grape phylloxera. Phylogenetic analyses showed that there are seven cytosolic DviGSTs and one microsomal DviGST. These cytosolic DviGSTs are clustered into four different classes including two delta genes, one omega gene, one theta gene, and three sigma genes. Among candidate cytosolic DviGSTs, a conserved N-terminal domain and a less conserved C-terminal domain were identified. For the candidate microsomal DviGST, three transmembrane regions were predicted. Multiple sequence alignment analysis of the candidate microsomal DviGST was conducted with other insect microsomal GSTs and the result showed that there is a conserved sequence pattern. Semiquantitative polymerase chain reaction was used to examine the tissue expression of these transcripts, and the results revealed that DviGSTs were ubiquitously expressed in the head and the body, but DviGSTd1, DviGSTd2, DviGSTs2, and DviGSTs3 were abundantly expressed in the head and body. This is the first study of the molecular characteristics of GST genes in grape phylloxera. Our results will provide a molecular basis for future studies of the detoxification mechanisms in grape phylloxera.