The apothecial ascomycete family Dactylosporaceae includes saprobes and lichenicolous fungi. In recent studies, the phylogenetic position of this family was unstable within the subphylum Pezizomycotina. The present study provides a stable phylogenetic placement for Dactylosporaceae within the class Eurotiomycetes and we introduce the new order: Dactylosporales Ekanayaka, E.B.G. Jones, Q. Zhao & K.D. Hyde, ord. nov. to accommodate this family. We also introduce two new species: Dactylospora chiangraiensis Ekanayaka, E.B.G. Jones, Q. Zhao & K.D. Hyde, sp. nov. and Dactylospora fusiformis Ekanayaka, E.B.G. Jones, Q. Zhao & K.D. Hyde, sp. nov. to this family and their relationships with other taxa are represented in a multigene phylogeny.