As a result of lichenological exploration in Ardahan (Turkey), thirty-four species of lichenicolous fungi belonging to 15 genera were determined on 20 different lichenized fungi. Three lichenicolous fungi — Intralichen lichenicola, Lichenoconium lichenicola, Polycoccum evae — are reported as new to Turkey and the Middle East. In addition Lichenostigma rupicolae, firstly reported from Turkey was also found on Pertusaria sp. for the second time in the world. Their geographical distribution are presented.