1Sl(1B) Chromosome substitution in Chinese Spring wheat promotes starch granule development and starch biosynthesis

Min Cao; Guanxing Chen; Chang Wang; Shoumin Zhen; Xiaohui Li; Wenying Zhang; F. J. Zeller; S. L. K. Hsam; Yingkao Hu; Yueming Yan

doi:10.1071/CP14256

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Min Cao,^1,* Guanxing Chen,^1,* Chang Wang,¹ Shoumin Zhen,¹ Xiaohui Li,¹ Wenying Zhang,² F. J. Zeller,³ S. L. K. Hsam,³ Yingkao Hu,^1,** Yueming Yanhttps://orcid.org/0000-0002-6985-0076^1,2,**

¹ACollege of Life Science, Capital Normal University, Beijing 100048, China.
²BHubei Collaborative Innovation Center for Grain Industry (HCICGI), Yangze University, Jingzhou 4340
³CDivision of Plant Breeding and Applied Genetics, Technical University of Munich, D-85354, Freising-

^*These authors contributed equally to this work.
^**Corresponding authors. Email: yingkaohu@yahoo.com; yanym@cnu.edu.cn

Abstract

The common wheat variety Chinese Spring (CS) chromosome substitution line CS-1S^l(1B) was used in this study, in which the 1B chromosome in CS (Triticum aestivum L., 2n = 6x = 42, AABBDD) was substituted by the 1S^l from Aegilops longissima (2n = 2x = 14, S^lS^l). The results showed that the substitution of 1B in CS by 1S^l chromosome could significantly increase amylopectin and total starch contents. The dynamic changes in starch granules during grain development in CS and CS-1S^l(1B) demonstrated that the substitution line possessed higher amount of A-type starch granules and greater diameter of both A- and B-granules. qRT-PCR revealed that some key genes involved in starch biosynthesis, such as starch synthases (SSI, SSII and SSIII), starch branching enzymes (SBE IIa and SBE IIb) and granule-bound starch synthase (GBSS I), displayed higher transcript levels of mRNA expressions during grain development in CS-1S^l(1B). Our results indicate that the substituted 1S^l chromosome carries important genes that influence starch granule development and starch biosynthesis, which may be used as potential gene resources for improvement of wheat starch quality.

Citation Download Citation

Min Cao, Guanxing Chen, Chang Wang, Shoumin Zhen, Xiaohui Li, Wenying Zhang, F. J. Zeller, S. L. K. Hsam, Yingkao Hu, and Yueming Yan "1S^l(1B) Chromosome substitution in Chinese Spring wheat promotes starch granule development and starch biosynthesis," Crop and Pasture Science 66(9), 894-903, (4 September 2015). https://doi.org/10.1071/CP14256

Received: 6 September 2014; Accepted: 1 April 2015; Published: 4 September 2015

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