Adult Black Redhorse (Moxostoma duquesnei) were seined from the Grand River, Ontario and artificially spawned in May 2007 and May 2008. Eggs hatched after 9–16 days at a mean temperature of 20°C, and after 11–25 days at a mean temperature of 17°C. Eggs did not develop fully at temperatures <11°C. Eggs and development of larvae between 9 and 24 mm TL, and juveniles up to 35 mm TL are described. Ontogeny of larval and juvenile Black Redhorse was compared to that of Greater Redhorse (Moxostoma valenciennesi), River Redhorse (Moxostoma carinatum), Golden Redhorse (Moxostoma erythrurum), Shorthead Redhorse (Moxostoma macrolepidotum), Copper Redhorse (Moxostoma hubbsi), and Spotted Sucker (Minytrema melanops). There was significant overlap between most meristic variables compared. However, the majority of Black Redhorse in this study (up to 18 mm TL) generally had higher myomere counts that were different from most other redhorse species. These data, in combination with knowledge of variation in congeneric distributions and differences in spawning windows, may allow identification of Black Redhorse as small as 9 mm TL.