Jupiaba poekotero, new species, is described from the Rio Tiquié, a tributary of the Rio Uaupés, in the upper Rio Negro system, estado do Amazonas, Brazil. It is distinguished from its congeners by the presence of an unpigmented, horizontally-oriented, roughly oval area on the proximal portion of the caudal-fin lobes, bordered posteriorly by dark pigmentation. Comments on the putative relationships of the new species are provided.