The deep-sea ceratioid anglerfish genus Acentrophryne is revised on the basis of all known material. Two species are recognized: the type species Acentrophryne longidens, represented by two specimens collected in the Gulf of Panama and from off the Pacific coast of Costa Rica and a new species described here on the basis of three specimens, all from off Peru. The new species differs from A. longidens in having a much longer distal escal appendage, a longer illicium, a narrower head, and fewer pectoral-fin rays. Diagnoses and descriptions are given for all taxa and a key to the species is provided.