During the period of 2003 to 2008, samples for isolation of dictyostelid cellular slime molds (dictyostelids) were collected from 17 localities throughout the state of Arkansas. The localities sampled included at least two examples in each of the six natural regions (Arkansas River Valley, Ozark Plateau, Ouachita Mountains, Gulf Coastal Plain, Crowley's Ridge and Mississippi Alluvial Plain) recognized for the State. The 167 samples collected from the 17 localities yielded a total of 2,082 individual clones representing 13 different species plus one form that could not be assigned to any described species. Six of the species recovered are reported from Arkansas for the first time, bringing the total number known from the State to 16. Polysphondylium pallidum was by far the most abundant species, comprising almost 50% of all isolates. Three other species (Dictyostelium minutum, P. violaceum and D. purpureum) made up approximately 30% of all remaining isolates, and the nine other species recovered were uncommon to rare. Two isolates of what appears to be Dictyostelium rhizopodium, a species in which the sorocarps have crampon bases, represent one of the northernmost known occurrences of a dictyostelid with this feature, which is characteristically found in species associated with tropical and subtropical regions of the world. Two species (D. caveatum and D. rosarium) reported previously from Arkansas were not isolated in the present study.